RT info:eu-repo/semantics/article
T1 Identification and quantification of carbamate pesticides in dried lime tree flowers by means of excitation-emission molecular fluorescence and parallel factor analysis when quenching effect exists
A1 Rubio Martínez, Laura
A1 Ortiz Fernández, Mª Cruz
A1 Sarabia Peinador, Luis Antonio
K1 Excitation-emission fluorescence
K1 Parallel factor analysis
K1 Carbamate pesticides
K1 Quenching effect
K1 Capability of detection
K1 Dried lime tree flowers
K1 Química analítica
K1 Chemistry, Analytic
AB A non-separative, fast and inexpensive spectrofluorimetric method based on the second order calibration of excitation-emission fluorescence matrices (EEMs) was proposed for the determination of carbaryl, carbendazim and 1-naphthol in dried lime tree flowers. The trilinearity property of three-way data was used to handle the intrinsic fluorescence of lime flowers and the difference in the fluorescence intensity of each analyte. It also made possible to identify unequivocally each analyte. Trilinearity of the data tensor guarantees the uniqueness of the solution obtained through parallel factor analysis (PARAFAC), so the factors of the decomposition match up with the analytes. In addition, an experimental procedure was proposed to identify, with three-way data, the quenching effect produced by the fluorophores of the lime flowers. This procedure also enabled the selection of the adequate dilution of the lime flowers extract to minimize the quenching effect so the three analytes can be quantified. Finally, the analytes were determined using the standard addition method for a calibration whose standards were chosen with a D-optimal design.The three analytes were unequivocally identified by the correlation between the pure spectra and the PARAFAC excitation and emission spectral loadings. The trueness was established by the accuracy line “calculated concentration versus added concentration” in all cases. Better decision limit values (CCα), in x0 = 0 with the probability of false positive fixed at 0.05, were obtained for the calibration performed in pure solvent: 2.97 μg L−1 for 1-naphthol, 3.74 μg L−1 for carbaryl and 23.25 μg L−1 for carbendazim. The CCα values for the second calibration carried out in matrix were 1.61, 4.34 and 51.75 μg L−1 respectively; while the values obtained considering only the pure samples as calibration set were: 2.65, 8.61 and 28.7 μg L−1, respectively.
PB Elsevier
SN 0003-2670
YR 2014
FD 2014-04
LK http://hdl.handle.net/10259/4258
UL http://hdl.handle.net/10259/4258
LA eng
NO theMinisteriodeEconomíayCompetitividad(CTQ2011-26022)and JuntadeCastillayLeón(BU108A11-2)
DS Repositorio Institucional de la Universidad de Burgos
RD 27-abr-2024