RT info:eu-repo/semantics/article T1 Effect of high pressure carbon dioxide processing on pectin methylesterase activity and other orange juice properties A1 Briongos Sánchez, Heliodoro A1 Illera Gigante, Alba Ester A1 Sanz Díez, Mª Teresa A1 Melgosa Gómez, Rodrigo A1 Beltrán Calvo, Sagrario A1 García Solaesa, Ángela K1 Orange juice K1 HPCD K1 Pectin methylesterase K1 Cloud stability K1 Chemical engineering K1 Ingeniería química AB Inactivation of pectinmethylesterase (PME) and quality parameters of orange juice have been studied after high pressure carbon dioxide (HPCD) treatment. The HPCD treatment conditions covered a wide range of temperature from 2 to 40 °C, far below normal thermal treatment, while operating pressure was varied from 10 to 30 MPa and exposure time from 3 to 60 min. A decrease in PME activity was found, even at the lowest temperature studied in this work, 2 °C. Different inactivation kinetic models were used to correlate the PME residual activity: the two-fraction model, the fractional-conversion model and the Weibull model. The two-fraction model presents the lowest mean relative deviation. Some quality parameters such as colour, pH, ºBrix, turbidity, ascorbic acid, total acidity and particle size distribution (PSD) were also determined right after HPCD treatment and along storage at 4 °C up to 12 days. PSD shows that HPCD treatment results in a volume increase of small particles and a volume decrease of large particles regarding the non-treated orange juice. Calcium content was also determined before and after HPCD treatment to check for insoluble calcium carbonate formation but not significant changes were observed in calcium content after HPCD treatment. PB Elsevier YR 2016 FD 2016-12 LK http://hdl.handle.net/10259/4344 UL http://hdl.handle.net/10259/4344 LA eng NO MINECO (CTQ2015-64396-R) DS Repositorio Institucional de la Universidad de Burgos RD 23-nov-2024