RT info:eu-repo/semantics/article
T1 Effect of Nanoparticles on Modified Screen Printed Inhibition Superoxide Dismutase Electrodes for Aluminum
A1 Barquero Quirós, Miriam
A1 Arcos Martínez, Julia
K1 superoxide dismutase biosensor
K1 aluminum
K1 tetrathiofulvalene
K1 screen-printed electrodes
K1 nanoparticles
K1 Chemistry, Analytic
K1 Química analítica
AB A novel amperometric biosensor for the determination of Al(III) based on the inhibitionof the enzyme superoxide dismutase has been developed. The oxidation signal of epinephrinesubstrate was affected by the presence of Al(III) ions leading to a decrease in its amperometric current.The immobilization of the enzyme was performed with glutaraldehyde on screen-printed carbonelectrodes modifiedwith tetrathiofulvalene (TTF) and different types ofnanoparticles. Nanoparticlesof gold, platinum, rhodium and palladium were deposited on screen printed carbon electrodesby means of two electrochemical procedures. Nanoparticles were characterized trough scanningelectronic microscopy, X-rays fluorescence, and atomic force microscopy. Palladium nanoparticlesshowed lower atomic force microscopy parameters and higher slope of aluminum calibration curvesand were selected to perform sensor validation. The developed biosensor has a detection limit of2.0   0.2  Mfor Al(III), with a reproducibility of 7.9% (n = 5). Recovery of standard reference materialspiked to buffer solution was 103.8% with a relative standard deviation of 4.8% (n = 5). Recovery oftap water spiked with the standard reference material was 100.5 with a relative standard deviation of3.4% (n = 3). The study of interfering ions has also been carried out.
PB MDPI
SN 1424-8220
YR 2016
FD 2016-10
LK http://hdl.handle.net/10259/4378
UL http://hdl.handle.net/10259/4378
LA eng
NO Vice-presidency for Research at theUniversity of Costa Rica (Project 804-B5-117), Ministerio de Ciencia e Innovación (MICINN Spain) and FondoEuropeo de Desarrollo Regional (FEDER) (Projects :TEC-TEC20013-40561-P and MUSSEL RTC-2015-4077-2).
DS Repositorio Institucional de la Universidad de Burgos
RD 19-abr-2024