RT info:eu-repo/semantics/article
T1 A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-L-Ascorbic Acid Trisodium Salt as a Substrate
A1 Alvarado Gámez, Ana Lorena
A1 Alonso Lomillo, Mª Asunción
A1 Domínguez Renedo, Olga
A1 Arcos Martínez, Julia
K1 alkaline phosphatase
K1 chronoamperometry
K1 2-phospho-L-ascorbic acid trisodium salt
K1 screen printed carbon electrode
K1 tungsten
K1 Chemistry, Analytic
K1 Química analítica
AB This paper presents a chronoamperometric method to determine tungsten in water using screen-printed carbon electrodes modified with gold nanoparticles and cross linked alkaline phosphatase immobilized in the working electrode. Enzymatic activity over 2-phospho-L-ascorbic acid trisodium salt, used as substrate, was affected by tungsten ions, which resulted in a decrease of chronoamperometric current, when a potential of 200 mV was applied on 10 mM of substrate in a Tris HCl buffer pH 8.00 and 0.36 M of KCl. Calibration curves for the electrochemical method validation, give a reproducibility of 5.2% (n = 3), a repeatability of 9.4% (n = 3) and a detection limit of 0.29 ± 0.01 μM. Enriched tap water, purified laboratory water and bottled drinking water, with a certified tungsten reference solution traceable to NIST, gave a recovery of 97.1%, 99.1% and 99.1% respectively (n = 4 in each case) and a dynamic range from 0.6 to 30 μM. This study was performed by means of a Lineweaver–Burk plot, showing a mixed kinetic inhibition.
PB MDPI
YR 2015
FD 2015-02
LK http://hdl.handle.net/10259/4379
UL http://hdl.handle.net/10259/4379
LA eng
NO Vicerrectorìa de Investigación de la Universidad de Costa Rica (Project 804-B2-297), via Junta de Castilla y León (BU212A12-2), Ministerio de Ciencia e Innovación (MICINN) (TEC2009-12029) and MICINN and Fondo Europeo de Desarrollo Regional (FEDER) (INNPACTO SERIBIO 2011-2014).
DS Repositorio Institucional de la Universidad de Burgos
RD 25-abr-2024