RT info:eu-repo/semantics/article
T1 Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media
A1 Barquero Quirós, Miriam
A1 Domínguez Renedo, Olga
A1 Alonso Lomillo, Mª Asunción
A1 Arcos Martínez, Julia
K1 acetylcholinesterase
K1 biosensor
K1 aluminum
K1 acetylthiocholine iodide
K1 screen-printed electrodes
K1 gold nanoparticles
K1 Chemistry, Analytic
K1 Química analítica
AB A novel amperometric biosensor for the determination of Al(III) based on the inhibition of the enzyme acetylcholinesterase has been developed. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles. The oxidation signal of acetylthiocholine iodide enzyme substrate was affected by the presence of Al(III) ions leading to a decrease in the amperometric current. The developed system has a detection limit of 2.1 ± 0.1 μM for Al(III). The reproducibility of the method is 8.1% (n = 4). Main interferences include Mo(VI), W(VI) and Hg(II) ions. The developed method was successfully applied to the determination of Al(III) in spiked tap water . The analysis of a certified standard reference material was also carried out. Both results agree with the certified values considering the respective associated uncertainties.
PB MDPI
YR 2014
FD 2014-05
LK http://hdl.handle.net/10259/4380
UL http://hdl.handle.net/10259/4380
LA eng
NO Research Vicerrectory of Costa Rica University (Project 804-B0-197), Junta de Castilla y León (BU212A12-2), Ministerio de Ciencia e Innovación (MICINN) and Fondo Europeo de Desarrollo Regional (FEDER) (INNPACTO SERIBIO 2011–2014) and TEC-2009/12029
DS Repositorio Institucional de la Universidad de Burgos
RD 04-dic-2024