RT info:eu-repo/semantics/article
T1 A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination
A1 Alvarado Gámez, Ana Lorena
A1 Alonso Lomillo, Mª Asunción
A1 Domínguez Renedo, Olga
A1 Arcos Martínez, Julia
K1 alkaline phosphatase
K1 biosensor
K1 gold nanoparticles
K1 4-nitrophenyl phosphate
K1 screen printed electrode
K1 vanadium
K1 water analysis
K1 Chemistry, Analytic
K1 Química analítica
AB A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water
PB MDPI
YR 2014
FD 2014-02
LK http://hdl.handle.net/10259/4381
UL http://hdl.handle.net/10259/4381
LA eng
NO Research Vicerrectory of Costa Rica University (Project 804-B0-058) and Spanish Ministry of Science and Innovation (TEC-2009/12029). This work was supported by the Spanish Ministry of Science and Innovation (MICINN) 410 and the European Regional Development Fund (FEDER) (INNPACTO SERIBIO 2011-411 2014) and TEC2009-12029, as well as through Junta de Castilla y León (BU212A12-2).
DS Repositorio Institucional de la Universidad de Burgos
RD 20-abr-2024