RT info:eu-repo/semantics/article
T1 Characterization and antimicrobial susceptibility of one antibiotic-sensitive and one multidrug-resistant Corynebacterium kroppenstedtii strain isolated from patients with granulomatous mastitis
A1 Fernández Natal, Isabel
A1 Rodríguez Lázaro, David
A1 Marrodán Ciordia, T.
A1 Sáez Nieto, J. A.
A1 Valdezate Ramos, S.
A1 Rodríguez Pollán, R. H.
A1 Tauch, A.
A1 Soriano, F.
K1 Antimicrobial susceptibility
K1 Corynebacterium kroppenstedtii
K1 granulomatous mastitis
K1 MALDI-TOF MS
K1 multidrug-resistant isolate
K1 Microbiology
K1 Microbiología
AB Human infections associated with Corynebacterium kroppenstedtii are rarely reported, and this organism is usually described as antibiotic sensitive. Almost all published cases of C. kroppenstedtii infections have been associated with breast pathology in women and have been described in New Zealand, France, Canada, India and Japan. Here we describe the microbiologic characteristics of two strains isolated from two women diagnosed of granulomatous mastitis in Spain. One C. kroppenstedtii isolate was antibiotic sensitive while the other was multidrug resistant. Biochemical identification was possible using a wide battery of methods including API Coryne V2.0, API Strep, API NH, API NE, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene amplification and sequencing. Antimicrobial susceptibility to 28 antibiotics as determined by Etest showed one isolate being sensitive to benzylpenicillin, ciprofloxacin, moxifloxacin, gentamicin, vancomycin, clindamycin, tetracycline, linezolid and rifampin. The second isolate showed resistance to ciprofloxacin, moxifloxacin, clindamycin, tetracycline and rifampin. The multidrug-resistant isolate contained the erm(X), tet(W), cmx, aphA1-IAB, strAB and sul1 resistance genes known from the R plasmid pJA144188 of Corynebacterium resistens. These genes were absent in the genome of the antibiotic-sensitive isolate. This report confirms the tropism of this microorganism for women's breasts and presents the first description of a multidrug-resistant C. kroppenstedtii strain.
PB Elsevier
YR 2016
FD 2016-11
LK http://hdl.handle.net/10259/4398
UL http://hdl.handle.net/10259/4398
LA eng
NO Gerencia Regional de Salud, Junta de Castilla y León, Spain, research project GRS 698/A/2011
DS Repositorio Institucional de la Universidad de Burgos
RD 22-dic-2024