RT info:eu-repo/semantics/article
T1 Supercritical carbon dioxide as solvent in the lipase-catalyzed ethanolysis of fish oil: kinetic study
A1 Melgosa Gómez, Rodrigo
A1 Sanz Díez, Mª Teresa
A1 García Solaesa, Ángela
A1 Paz Barragán, Esther de
A1 Beltrán Calvo, Sagrario
A1 Lamas, Daniela L.
K1 Omega 3
K1 Lipase
K1 Ethanolysis
K1 Supercritical carbon dioxide
K1 Chemical engineering
K1 Ingeniería química
AB Supercritical carbon dioxide (SC-CO2) has been used as green solvent in the lipase-catalyzed ethanolysis of fish oil by Lipozyme RM IM at mild, non-oxidative conditions and with no solvent residues. The effect of experimental conditions, initial substrate ethanol/oil molar ratio (2–38), pressure (7.5–30 MPa), and temperature (323.15–353.15 K) on equilibrium conversion, reaction rate and oxidative status of the products has been studied. No ethanol inhibition has been observed at high concentrations of ethanol, when putting in contact first the fish oil with the enzyme avoiding direct contact between the biocatalyst and ethanol. Operating pressure affected positively the reaction performance in the range investigated. Visual observation of the phase behaviour of the initial reaction mixture showed an “expanded liquid phase” that helped enhancing reaction rate, and a gas phase. Raising temperature accelerated the reaction up to a limit (343.15 K), observing higher enzyme thermal stability than in other reaction media (313.15 K). However, lipid oxidation increases with temperature. Up to 86 ± 1% FAEE yield has been found at MR = 6:1, 30 MPa and 323.15 K. Kinetic data have been correlated by using a mathematical model based on the elementary reactions of the 3-step transesterification. Kinetic rate constants, apparent activation volumes and energies are reported for the first time for a lipase-catalyzed ethanolysis reaction in SC-CO2.
PB Elsevier
SN 2212-9820
YR 2017
FD 2017-01
LK http://hdl.handle.net/10259/4567
UL http://hdl.handle.net/10259/4567
LA eng
NO Spanish Government (MINECO) and the EuropeanRegional Development Fund (ERDF) forfinancial support of theproject CTQ2012-39131-C02-01. Financial support from the Juntade Castilla y León and European Regional Development Fund(ERDF) through project BU055U16
DS Repositorio Institucional de la Universidad de Burgos
RD 23-nov-2024