RT info:eu-repo/semantics/article
T1 Comparing ancient DNA survival and proteome content in 69 archaeological cattle tooth and bone samples from multiple European sites
A1 Wadsworth, Caroline .
A1 Procopio, Noemi .
A1 Anderung, Cecilia .
A1 Carretero Díaz, José Miguel
A1 Iriarte Avilés, Eneko
A1 Valdiosera Morales, Cristina Eugenia
A1 Rengert Elburg .
A1 Kirsty Penkman .
A1 Buckley, Michael
K1 Ancient DNA
K1 Ancient proteins
K1 Proteomics
K1 Collagen
K1 Non-collagenous proteins
K1 Paleontology
K1 Paleontología
AB Ancient DNA (aDNA) is themost informative biomolecule extracted fromskeletal remains at archaeological sites,but its survival is unpredictable and its extraction and analysis is time consuming, expensive and often fails. Severalproposed methods for better understanding aDNA survival are based upon the characterisation of some aspectof protein survival, but these are typically non-specific; proteomic analyses may offer an attractive methodfor understanding preservation processes. In this study, in-depth proteomic (LC-Orbitrap-MS/MS) analyseswerecarried out on 69 archaeological bovine bone and dentine samples from multiple European archaeological sitesand comparedwith mitochondrial aDNA and amino acid racemisation (AAR) data. Comparisons of these data, includingestimations of the relative abundances for seven selected non-collagenous proteins, indicate that the survivalof aDNA in bone or dentine may correlatewith the survival of some proteins, and that proteome complexityis a more useful predictor of aDNA survival than protein abundance or AAR. The lack of a strong correlation betweenthe recovery of aDNA and the proteome abundance may indicate that the survival of aDNA is more closelylinked to its ability to associate with bone hydroxyapatite crystals rather than to associate with proteins.Significance: Ancient biomolecule survival remains poorly understood, even with great advancements in ‘omics’technologies, both in genomics and proteomics. This study investigates the survival of ancient DNA in relation tothat of proteins, taking into account proteome complexity and the relative protein abundances to improve ourunderstanding of survival mechanisms. The results show that although protein abundance is not necessarily directlyrelated to aDNA survival, proteome complexity appears to be.
PB Elsevier
SN 1874-3919
YR 2017
FD 2017-03
LK http://hdl.handle.net/10259/4724
UL http://hdl.handle.net/10259/4724
LA eng
NO NERC for funding the studentship to CW (NE/J500057/1) and the Royal Society for funding for MB (UF120473 and UF120473). Assistant Professor (CA) funded by the Swedish Research Council Formas (2010-627) and KP by the Leverhulme Trust (PLP-2012-116). The Atapuerca research was supported by the Spanish MINECO (CGL2012-38434-C03-01) project. Fieldwork at the Atapuerca sites was funded by the Junta de Castilla y León and the Fundación Atapuerca.
DS Repositorio Institucional de la Universidad de Burgos
RD 23-nov-2024