RT info:eu-repo/semantics/article T1 Effect of thermosonication batch treatment on enzyme inactivation kinetics and other quality parameters of cloudy apple juice A1 Illera Gigante, Alba Ester A1 Sanz Díez, Mª Teresa A1 Benito Román, Oscar A1 Varona Fernández, Sandra A1 Beltrán Calvo, Sagrario A1 Melgosa Gómez, Rodrigo A1 García Solaesa, Ángela K1 Cloudy apple juice K1 Thermosonication K1 Enzyme inactivation K1 Dissolved gases K1 Chemical engineering K1 Ingeniería química AB Cloudy apple juice has been treated by thermosonication in batch mode as an alternative processing to thermal treatment. Thermosonication was found to be effective to inactivate polyphenol oxidase; however, pectinmethylesterase was found to be more resistant. An increase of the working ultrasound amplitude and the amount of energy supplied to the juice led to lower enzyme residual activities.Enzyme inactivation kinetics were determined at different temperatures (from 44 to 67 °C). Inactivation data were described by the first order kinetic model and the Weibull model, both models yielding good fitting. Thermosonication treatment caused a homogenization effect reflected in the shifting of the particle size distribution towards smaller diameters. The effect of the nature of dissolved gases in the juice on enzyme inactivation was studied by displacing the air dissolved in the juice by bubbling nitrogen or carbon dioxide, previous to the thermosonication experiments. Higher inactivation rates were obtained by displacing the air with nitrogen.Industrial relevanceConsumers demand of natural and fresh-like products has driven the food industry to investigate alternative technologies to replace conventional food heat treatments that may affect food quality. Among these technologies, thermosonication treatment is an attractive technology that can inactivate microorganisms and enzymes. This work shows that some enzymes that cause deleterious effect on cloudy apple juice can be more effectively inactivated by thermosonication than by thermal treatment, in the same temperature range, reducing the damages caused by heating. PB Elsevier SN 1466-8564 YR 2018 FD 2018-06 LK http://hdl.handle.net/10259/4744 UL http://hdl.handle.net/10259/4744 LA eng NO Spanish Government (MINECO) and the European RegionalDevelopment Fund (ERDF) for financial support of project CTQ2015-64396-R and AEI's contract. To MINECO for RM's pre-doctoral contract(BES-2013-063937). To University of Burgos for AGS's pre-doctoralcontract. To Consejería de Educación, Junta de Castilla y León andERDF for financial support of project BU055U16 and O. Benito Román´sPost-doctoral contract. DS Repositorio Institucional de la Universidad de Burgos RD 27-dic-2024