RT info:eu-repo/semantics/article
T1 Potassium‐ion‐selective fluorescent sensors to detect cereulide, the emetic toxin of B. cereus, in food samples and HeLa cells
A1 García Calvo, José
A1 Ibeas Cortes, Saturnino
A1 Antón García, Eva Clara
A1 Torroba Pérez, Tomás
A1 González Aguilar, Gerardo .
A1 Antunes, Wilson .
A1 González Lavado,  Eloísa .
A1 Fanarraga, Mónica L. .
K1 Chemistry
K1 Química
AB We report the development of new chemical probes for cereulide, a toxic metabolite produced by specific strains of Bacillus cereus, through displacement of potassium cations from a preformed specific complex and a subsequent change in the fluorescence emission. For this purpose, we designed fluorescent probes for potassium cations that were suitable for displacement assays with cereulide from organic extracts. The fluorescence detection of natural cereulide in rice samples was achieved by using synthetic cereulide as a reference and a potassium fluorescent reporter, and this was found to be useful as a portable and fast method for the in situ detection of cereulide in food extracts. To study the fate of cereulide in live cells, we designed a procedure that was suitable for live‐cell microscopy imaging of HeLa cells by comparing the cellular location of the potassium fluorogenic probe, which stained intracellular endolysosomes, in the absence and presence of cereulide; we concluded that in the presence of cereulide, the fluorescence of the probe was decreased because of complexation of the potassium ions by cereulide.
PB Wiley-VCH Verlag
SN 2191-1363
YR 2017
FD 2017-08
LK http://hdl.handle.net/10259/4776
UL http://hdl.handle.net/10259/4776
LA eng
NO Ministeriode Econom&a y Competitividad, Spain (Projects CTQ2015-71353-Rand AES-PI16/000496), Junta de Castilla y Lejn, Consejer&a de Educacijn y Cultura y Fondo Social Europeo (Project BU232U13),and the European Commission, Seventh Framework Programme(Project SNIFFER FP7-SEC-2012–312411)
DS Repositorio Institucional de la Universidad de Burgos
RD 22-dic-2024