RT info:eu-repo/semantics/article
T1 Fishing for G-Quadruplexes in solution with a perylene diimide derivative labeled with biotins
A1 Busto Vázquez, Natalia
A1 Calvo Gredilla, Patricia
A1 Santolaya, Javier
A1 Leal Villalba, José María
A1 Guédin, Aurore .
A1 Barone, Giampaolo
A1 Torroba Pérez, Tomás
A1 Mergny, Jean-Louis
A1 García Ruiz, Begoña
K1 Química
K1 Chemistry
AB A new fluorescent, non‐cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G‐quadruplexes with scarce or no affinity towards single‐ or double‐stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G‐quadruplex structures in solution by use of PDI2B and streptavidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G‐quadruplex structures from solution, even in the presence of an excess of single‐stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2‐ over 3‐tetrad quadruplexes, which constitutes a novel type of behavior.
PB Wiley-VCH Verlag
SN 0947-6539
YR 2018
FD 2018-08
LK http://hdl.handle.net/10259/4882
UL http://hdl.handle.net/10259/4882
LA eng
NO “la Caixa” Foundation (LCF/PR/PR12/11070003), MINECO (CTQ2014-58812-C2-2-R and CTQ2015-71353-R, FEDER Funds), and Junta de Castilla y Lejn, Consejer&a de Educacijn y Cultura y Fondo Social Europeo (ProjectBU051U16), Spain, is gratefully acknowledged. N.B. is gratefulto Dr. Oscar Mendoza of the ARNA Laboratory for useful discussionand also to the financial support of the Jos8 CastillejoProgram by the Spanish Ministry of Education, Culture andSports (JC2015-00403). J.L.M. acknowledges support from ConseilRegional d’Aquitaine, Agence Nationale de la Recherche(ANR Quarpdiem) and the Symbit program [CZ.02.1.01/0.0/0.0/15_003/0000477] financed by the ERDF.
DS Repositorio Institucional de la Universidad de Burgos
RD 22-dic-2024