RT info:eu-repo/semantics/article
T1 Binding studies of metal–salphen and metal–bipyridine complexes towards G‐Quadruplex DNA
A1 Łęczkowska, Anna .
A1 González García, Jorge .
A1 Pérez Arnáiz, Cristina
A1 García Ruiz, Begoña
A1 White, Andrew J. P. .
A1 Vilar, Ramón .
K1 cancer
K1 DNA binding
K1 heterocycles
K1 metal complexes
K1 polymerase
K1 Química física
K1 Chemistry, Physical and theoretical
AB The proposed in vivo formation of G-quadruplexDNA (G4 DNA) in promoter regions of oncogenes and in telomereshas prompted the development of small moleculeswith high affinity and selectivity for these structures. Hereinwe report the synthesis of a new di-substituted bipyridineligand and the corresponding complexes with Ni2+ andVO2+. Both these new complexes have been characterizedspectroscopically and by X-ray crystallography. Detailed DNAbinding studies of these two complexes, together with threepreviously reported metal salphen complexes, are presented.Using FRET melting assays, the binding affinity and selectivityof the five metal complexes against six different G4 DNAstructures as well as a duplex DNA have been determined.In addition, we present detailed ITC and UV/Vis studies tocharacterize the interaction of the complexes with humantelomeric G4 DNA. Finally, we show via a polymerase stopassay that these complexes are able to stabilize a G4 DNAstructure (from the c-Myc oncogene promoter) and halt theactivity of Taq polymerase.
PB Wiley-VCH Verlag
SN 0947-6539
YR 2018
FD 2018-08
LK http://hdl.handle.net/10259/4911
UL http://hdl.handle.net/10259/4911
LA eng
NO UK’s Engineering and Physical Sciences Research Council(EPSRC) (grant number: EP/H005285/1)
DS Repositorio Institucional de la Universidad de Burgos
RD 04-dic-2024