RT info:eu-repo/semantics/article
T1 Pectin methylesterase inactivation by High Pressure Carbon Dioxide (HPCD)
A1 Benito Román, Oscar
A1 Sanz Díez, Mª Teresa
A1 Illera Gigante, Alba Ester
A1 Melgosa Gómez, Rodrigo
A1 Benito Moreno, José Manuel
A1 Beltrán Calvo, Sagrario
K1 PME
K1 HPCD
K1 Enzyme inactivation
K1 CO2
K1 Juice
K1 Ingeniería química
K1 Chemical engineering
AB In the present work the effect of High Pressure Carbon Dioxide (HPCD) on the activity and structure of a commercial pectin methylesterase (PME) was studied and the results were compared to the conventional thermal inactivation technology. The use of supercritical CO2 (pressure 6–18 MPa, temperature 40–55 °C and time up to 75 min) increased dramatically the PME inactivation rate. The experimental data fitted the first order model and the inactivation kinetic study of PME was completed with the calculations of the activation energy and volume of activation.HPCD treatment induced important changes in the tertiary structure of the enzyme, as revealed the intrinsic fluorescence, KI quenching and ANS binding analyses. They showed that HPCD treatment rearranged the structure of the enzyme. These changes induced by HPCD were significantly different from those induced by mild thermal treatment. Finally, the structural changes detected correlated with the enzyme activity losses observed.
PB Elsevier
SN 0896-8446
YR 2019
FD 2019-03
LK http://hdl.handle.net/10259/5045
UL http://hdl.handle.net/10259/5045
LA eng
NO Spanish Ministry of Economy and Competitiveness for financial support of project CTQ2015-64396-R and A.E. Illera’s contract. To Junta de Castilla y León and ERDF for financial support of project BU055U16 and O. Benito-Román’s Post-doctoral contract. To Spanish Mi
DS Repositorio Institucional de la Universidad de Burgos
RD 25-abr-2024