RT info:eu-repo/semantics/article
T1 Signal transfer with excitation-emission matrices between a portable fluorimeter based on light-emitting diodes and a master fluorimeter
A1 Sanllorente Méndez, Silvia
A1 Rubio Martínez, Laura
A1 Ortiz Fernández, Mª Cruz
A1 Sarabia Peinador, Luis Antonio
K1 Signal transfer
K1 Portable fluorimeter
K1 Excitation-emission fluorescence
K1 PARAFAC
K1 Capability of detection
K1 Enrofloxacin
K1 Química analítica
K1 Chemistry, Analytic
AB In this work, the transfer of the excitation-emission matrices between a portable fluorimeter based on LEDs and a master fluorimeter based on a xenon source was carried out. Enrofloxacin was the analyte of interest and it was measured alone or in binary mixtures with flumequine (partially overlapped signals) or with ciprofloxacin (fully overlapped signals). The maintenance and transfer of the unequivocal identification of the fluorophores between both instruments are shown. The precision in the determination performed with the portable fluorimeter approximated to that made with the master fluorimeter using this transfer and it did not introduce bias. The correlation coefficients of the calibrations based on PARAFAC using EEM signals were higher than 0.999, whereas the values of the capability of detection ranged from 14.8 to 26.9 μg L−1 for probabilities of false positive and false negative fixed at 0.05. These results contribute to the effort to perform the fluorimetric detection outside the laboratory and to promote the use of databases of fluorescence spectra for the unequivocal identification in remote of fluorophores of interest and/or regulated.
PB Elsevier
SN 0925-4005
YR 2019
FD 2019-04
LK http://hdl.handle.net/10259/5058
UL http://hdl.handle.net/10259/5058
LA eng
NO Spanish MINECO (AEI/FEDER, UE) through projects CTQ2014-53157-R and CTQ2017‐88894‐R and by Junta de Castilla y León through project BU012P17 (all co‐financed with European FEDER funds)
DS Repositorio Institucional de la Universidad de Burgos
RD 25-abr-2024