RT info:eu-repo/semantics/article T1 Effect of High Pressure Carbon Dioxide on polyphenoloxidase from Litopenaeus vannamei A1 Illera Gigante, Alba Ester A1 Sanz Díez, Mª Teresa A1 Beltrán Calvo, Sagrario A1 Benito Román, Oscar K1 Litopenaeus vannamei K1 HPCD K1 Solid samples K1 PPO inactivation K1 Ingeniería química K1 Chemical engineering AB The extraction of polyphenoloxidase (PPO) from Litopenaeus vannamei was studied at different temperatures, pH and ionic strength conditions. Optimum extracts at pH = 7.2 were treated by hiph pressure carbon dioxide (HPCD) technology at 20 MPa and in a temperature range from 35 to 50 °C. Inactivation kinetics for PPO extracts were determined and fitted to the Weibull model. The effect of HPCD on PPO activity was also evaluated in shrimps in vivo, finding that PPO could be easier inactivated than in the crude PPO extract. Color of Litopenaeus vannamei was visually followed right after HPCD treatment and thermal treatment at the same temperature and during storage at 4 °C during a week, showing good results when shrimps were treated with HPCD in the absence of water. PB Elsevier SN 0023-6438 YR 2019 FD 2019-07 LK http://hdl.handle.net/10259/5091 UL http://hdl.handle.net/10259/5091 LA eng NO Spanish Government (MINECO) and the European Regional Development Fund for financial support of project CTQ2015-64396-R DS Repositorio Institucional de la Universidad de Burgos RD 23-nov-2024