RT info:eu-repo/semantics/article
T1 Biological activity and photocatalytic properties of a naphthyl-imidazo phenanthroline (HNAIP) ligand and its [Ir(ppy)2(HNAIP)]Cl and [Rh(ppy)2(HNAIP)]Cl complexes
A1 Rubio Antolin, Ana Rosa
A1 Fidalgo Zorrilla, Jairo
A1 Martin Vargas, Judit
A1 Pérez Arnáiz, Cristina
A1 Alonso de la Torre, Sara
A1 Biver, Tarita
A1 Espino Ordóñez, Gustavo
A1 Busto Vázquez, Natalia
A1 García Ruiz, Begoña
K1 2-(hydroxy-1-naphtyl)imidazo-[4,5-f]
K1 [1,10]phenanthroline
K1 Iridium
K1 Rhodium
K1 Cellular test
K1 DNA
K1 Photo-catalysis
K1 Bioquímica
K1 Biochemistry
K1 Química inorgánica
K1 Chemistry, Inorganic
AB The synthesized 2-(hydroxy-1-naphtyl)imidazo-[4,5-f][1,10]phenanthroline (HNAIP) ligand and its new iridium ([Ir(ppy)2(HNAIP)]Cl) and rhodium ([Rh(ppy)2(HNAIP)]Cl) complexes, being ppy = 2-phenylpiridinate, show cytotoxic effects in SW480 (colon adenocarcinoma) and A549 (epithelial lung adenocarcinoma) cells. They all are cytotoxic in the tested cell lines. HNAIP and [Rh(ppy)2(HNAIP)]+ are the most cytotoxic, whereas [Ir(ppy)2(HNAIP)]+ displays negligible cytotoxicity towards A549 cells and moderate activity towards SW480. The interaction of all three compounds with Bovine Serum Albumin (BSA), l-glutathione reduced (GSH), nicotinamide adenine dinucleotide (NADH) and DNA was studied to explain the differences found in terms of cytotoxicity. None of them are able to interact with BSA, thus excluding bioavailability due to plasma protein interaction as the possible differentiating factor in their biological activity. By contrast, small differences have been observed regarding DNA interaction. In addition, taking advantage of the emission properties of these molecules, they have been visualized in the cytoplasmic region of A549 cells. Inductively coupled plasma mass spectrometry (ICP-MS) experiments show, in turn, that the internalization ability follow the sequence [Rh(ppy)2(HNAIP)]+ > [Ir(ppy)2(HNAIP)]+ > cisplatin. Therefore, it seems clear that the cellular uptake by tumour cells is the key factor affecting the different cytotoxicity of the metal complexes and that this cellular uptake is influenced by the hydrophobicity of the studied complexes. On the other hand, preliminary catalytic experiments performed on the photo-oxidation of GSH and some amino acids such as l-methionine (Met), l-cysteine (Cys) and l-tryptophan (Trp) provide evidence for the photocatalytic activity of the Ir(III) complex in this type of reactions.
PB Elsevier
SN 0162-0134
YR 2020
FD 2020-02
LK http://hdl.handle.net/10259/5194
UL http://hdl.handle.net/10259/5194
LA eng
NO “la Caixa” Banking Foundation (LCF/PR/PR12/11070003), Ministerio de Ciencia, Innovación y Universidades (RTI2018-102040-B-100 and RTI2018-100709-B-C21), Junta de Castilla y León (BU305P18, FEDER Funds)
DS Repositorio Institucional de la Universidad de Burgos
RD 22-dic-2024