RT info:eu-repo/semantics/article
T1 Thiosemicarbazone-metal complexes exhibiting cytotoxicity in colon cancer cell lines through oxidative stress
A1 Alcaraz, Raquel
A1 Muñiz Rodríguez, Pilar
A1 Cavia Saiz, Mónica
A1 Palacios, Óscar
A1 Samper, Katia G.
A1 Gil García, Rubén
A1 Jiménez Pérez, Alondra
A1 García Tojal, Javier
A1 García Girón, Carlos
K1 Cell death
K1 Cobalt compounds
K1 Colon carcinoma
K1 Iron compounds
K1 Oxidative stress
K1 Thiosemicarbazone
K1 Bioquímica
K1 Biochemistry
AB Colorectal cancer is the third most common type of cancer and has a high incidence in developed countries. At present, specific treatments are being required to allow individualized therapy depending on the molecular alteration on which the drug may act. The aim of this project is to evaluate whether HPTSC and HPTSC* thiosemicarbazones (HPTSC = pyridine-2-carbaldehyde thiosemicarbazone and HPTSC* = pyridine-2-carbaldehyde 4N-methylthiosemicarbazone), and their complexes with different transition metal ions as Cu(II), Fe(III) and Co(III), have antitumor activity in colon cancer cells (HT-29 and SW-480), that have different oncogenic characteristics. Cytotoxicity was evaluated and the involvement of oxidative stress in its mechanism of action was analyzed by quantifying the superoxide dismutase activity, redox state by quantification of the thioredoxin levels and reduced/oxidized glutathione rate and biomolecules damage. The apoptotic effect was evaluated by measurements of the levels of caspase 9 and 3 and the index of histones. All the metal-thiosemicarbazones have antitumor activity mediated by oxidative stress. The HPTSC*-Cu was the compound that showed the best antitumor and apoptotic characteristics for the cell line SW480, that is KRAS gene mutated.
PB Elsevier
SN 0162-0134
YR 2020
FD 2020-05
LK http://hdl.handle.net/10259/5318
UL http://hdl.handle.net/10259/5318
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 23-nov-2024