RT info:eu-repo/semantics/article
T1 Polyphenol oxidase (PPO) and pectin methylesterase (PME) inactivation by high pressure carbon dioxide (HPCD) and its applicability to liquid and solid natural products
A1 Benito Román, Oscar
A1 Sanz Díez, Mª Teresa
A1 Illera Gigante, Alba Ester
A1 Melgosa Gómez, Rodrigo
A1 Beltrán Calvo, Sagrario
K1 PPO
K1 PME
K1 Juice
K1 Supercritical carbon dioxide
K1 Green process
K1 Food technology
K1 Ingeniería química
K1 Chemical engineering
AB In the present work, High Pressure Carbon Dioxide (HPCD) has been used to inactivate the enzymes Polyphenol Oxidase (PPO) and Pectin Methylesterase (PME). The work has been done in two stages: in the first one, fundamental knowledge about the inactivation mechanism induced by HPCD was obtained using pure commercial enzymes. The influence of the main process parameters was studied: pressure, temperature, time and ratio CO2/volume of enzymatic solution. The changes in the tertiary structure of the enzymes after the HPCD treatment were analyzed by fluorescence spectroscopy running two different tests: intrinsic fluorescence measurement and ANS binding. In the second stage, the acquired knowledge was used to study the inactivation pattern of these two enzymes in liquid and solid natural products, including fruit and vegetable juices or shrimps. The results revealed differences in the inactivation pattern of the enzymes in these substrates compared to the pure commercial enzymes, but the applicability of HPCD technology for the inactivation of enzymes in real substrates at industrial scale was also revealed.
PB Elsevier
SN 0920-5861
YR 2020
FD 2020-04
LK http://hdl.handle.net/10259/5327
UL http://hdl.handle.net/10259/5327
LA eng
NO Spanish Government (MINECO) and the European Regional Development Fund (ERDF) for financial support of project CTQ2015-64396-R and A.E. Illera’s contract. To Junta de Castilla y León and ERDF for financial support of project BU055U16 and O. Benito-Román’s Post-doctoral contract.
DS Repositorio Institucional de la Universidad de Burgos
RD 24-dic-2024