RT info:eu-repo/semantics/article T1 Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization A1 Osés Gómez, Sandra María A1 Marcos, Patricia A1 Azofra, Patricia A1 Pablo, Ana de A1 Fernández Muiño, Miguel Ángel A1 Sancho Ortiz, María Teresa K1 propolis K1 phenolic profile K1 antioxidant capacities K1 hyaluronidase inhibitory activity K1 ACE inhibitory activity K1 Alimentos K1 Food AB Propolis is a resinous vegetal exudate modified by bees, and is interesting as apreservative and potentially functional product. This work dealt with studying the commonphenolic profiles and antioxidant capacities of 13 bee propolis from different geographical areas.Both hyaluronidase and angiotensin converting enzyme (ACE) inhibitory activities were alsoassessed and related when possible with particular phenolic compounds. High performance liquidchromatography‐ultraviolet detection (HPLC‐UV) analysis showed that every propolis containedp‐coumaric acid (1.2–12.2 mg/g) and ferulic acid (0.3–11.0 mg/g). Pinocembrin, catechin, and caffeicacid phenethyl ester (CAPE) plus galangin were the main flavonoids. Antioxidant activities werehigher than 280 μmol trolox/g for trolox equivalent antioxidant capacity (TEAC), 0.099 mmol uricacid/g for radical‐scavenging effect on hydroxyl radicals, and 0.19 mg/mL for half maximalinhibitory concentration (IC50) of antioxidant activity against superoxide anion radical. Workingwith solutions of 10 mg/mL propolis, hyaluronidase inhibitory activity ranged between 0% and68.20%, being correlated to ferulic acid content. ACE inhibitory effect determined by HPLC washigher than 78%, being correlated with catechin and p‐coumaric acid. Therefore, propolis could beuseful for food, pharmaceutical, and cosmetic companies, also helping to reduce risk factors fordiseases related to oxidative damage, inflammatory processes, and hypertension. This research alsohighlights the necessity for harmonized analysis methods and the expression of results for propolis. PB MDPI YR 2020 FD 2020-01 LK http://hdl.handle.net/10259/5367 UL http://hdl.handle.net/10259/5367 LA eng DS Repositorio Institucional de la Universidad de Burgos RD 11-dic-2024