RT info:eu-repo/semantics/article T1 Determination of Volatiles in Mouse Urine by Headspace Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry A1 Arnáiz, Esther A1 Moreno Mediavilla, Daniel A1 Quesada Pato, Roberto K1 Experimental design K1 Gas chromatography-mass spectrometry (GC-MS) K1 Headspace-solid-phase micro-extraction (HS-SPME) K1 Mouse urine K1 Volatile organic compounds K1 Química orgánica K1 Chemistry, Organic AB The extraction and determination of volatile compounds in mice urine were performed using headspace solid-phase microextraction and gas chromatography-mass spectrometry. In order to optimize the extraction conditions, experimental design was applied. A sample volume of 108 µl, a temperature of 148.6°C, and a time of 94 minutes were found to be the optimal conditions. Samples of male and female mouse urine were analyzed to determine volatile compound profiles. A total of 36 organic compounds including ketones, aldehydes, and terpenes were detected. The results revealed that compounds such as 2-isopropyl-4,5-dihydrothiazole, which is considered a male sexual pheromone, were only detected in male urine samples, whereas others like benzaldehyde were especially abundant in female mouse urine. A comparison of female samples corresponding to different stages of the estrous cycle was also performed. PB Taylor & Francis Group SN 0003-2719 YR 2014 FD 2014-03 LK http://hdl.handle.net/10259/5371 UL http://hdl.handle.net/10259/5371 LA spa NO European Commission ([SME-2011-1] Research for SMEs, PiedPiper project, Grant agreement: 286852) DS Repositorio Institucional de la Universidad de Burgos RD 28-mar-2024