RT info:eu-repo/semantics/article
T1 Determination of Volatiles in Mouse Urine by Headspace Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry
A1 Arnáiz, Esther
A1 Moreno Mediavilla, Daniel
A1 Quesada Pato, Roberto
K1 Experimental design
K1 Gas chromatography-mass spectrometry (GC-MS)
K1 Headspace-solid-phase micro-extraction (HS-SPME)
K1 Mouse urine
K1 Volatile organic compounds
K1 Química orgánica
K1 Chemistry, Organic
AB The extraction and determination of volatile compounds in mice urine were performed using headspace solid-phase microextraction and gas chromatography-mass spectrometry. In order to optimize the extraction conditions, experimental design was applied. A sample volume of 108 µl, a temperature of 148.6°C, and a time of 94 minutes were found to be the optimal conditions. Samples of male and female mouse urine were analyzed to determine volatile compound profiles. A total of 36 organic compounds including ketones, aldehydes, and terpenes were detected. The results revealed that compounds such as 2-isopropyl-4,5-dihydrothiazole, which is considered a male sexual pheromone, were only detected in male urine samples, whereas others like benzaldehyde were especially abundant in female mouse urine. A comparison of female samples corresponding to different stages of the estrous cycle was also performed.
PB Taylor & Francis Group
SN 0003-2719
YR 2014
FD 2014-03
LK http://hdl.handle.net/10259/5371
UL http://hdl.handle.net/10259/5371
LA spa
NO European Commission ([SME-2011-1] Research for SMEs, PiedPiper project, Grant agreement: 286852)
DS Repositorio Institucional de la Universidad de Burgos
RD 23-nov-2024