RT info:eu-repo/semantics/article T1 Supercritical CO2 and subcritical water technologies for the production of bioactive extracts from sardine (Sardina pilchardus) waste A1 Melgosa Gómez, Rodrigo A1 Trigueros Andrés, Ester A1 Sanz Díez, Mª Teresa A1 Cardeira, Martim A1 Rodrigues, Liliana A1 Fernández, Naiara A1 Matias, Ana A. A1 Bronze, Maria Rosário A1 Marques, Marta A1 Paiva, Alexandre A1 Simões, Pedro K1 Sardine waste K1 Subcritical water K1 Fish protein hydrolysate K1 Biorefinery K1 Antioxidant activity K1 Antiproliferative activity K1 Ingeniería química K1 Chemical engineering K1 Alimentos K1 Food AB The valorization of sardine (Sardina pilchardus) waste (SW) from a canning facility has been investigated within a biorefining approach. Sequential fractionation of SW into its constituents has been carried out using green solvents such as supercritical carbon dioxide (SCCO2) and subcritical water (sCW). The lipid fraction has been isolated through supercritical fluid extraction (SFE) with SCCO2 at 250 bar and 40 °C, yielding 20.3 ± 0.2 g oil/100 g SW with up to 17.2 %wt. omega-3 polyunsaturated fatty acids (PUFAs). Aiming at the protein fraction, sCW extraction/hydrolysis has been carried out at different temperatures (90, 140, 190 and 250 °C), using both SW and defatted sardine waste (DSW) from SFE experiments. Previous defatting increased protein recovery and purity. Bioactive properties of the fish protein hydrolysates (FPHs) obtained were affected by the extraction temperature. The highest antioxidant activity and in vitro antiproliferative effect were found in the extracts obtained at 250 °C. PB Elsevier SN 0896-8446 YR 2020 FD 2020-10 LK http://hdl.handle.net/10259/5395 UL http://hdl.handle.net/10259/5395 LA eng NO FCT/MCTES (UIDB/QUI/50006/2020), and Fundação para a Ciência e a Tecnologia through project PTDC/ASP-PES/28399/2017 and grants IF/01146/2015 and SFRH/BD/116002/2016 DS Repositorio Institucional de la Universidad de Burgos RD 20-abr-2024