RT info:eu-repo/semantics/article
T1 Zn(II) detection in biological samples with a smart sensory polymer
A1 Guembe García, Marta
A1 Vallejos Calzada, Saúl
A1 Carreira Barral, Israel
A1 Ibeas Cortes, Saturnino
A1 García García, Félix Clemente
A1 Santaolalla García, Victoria
A1 Moradillo Renuncio, Natalia
A1 García Pérez, José Miguel
K1 Smart materials
K1 Sensory polymers
K1 Zinc(II) detection
K1 Biological samples
K1 Chronic wounds
K1 Química orgánica
K1 Chemistry, Organic
AB We have developed a new sensory material for the rapid and inexpensive determination of Zn(II), and we have carried out a proof of concept for the determination of Zn(II) in biological samples. The interaction with Zn(II) generates an OFF-ON fluorescence process on the material, which can be recorded both with a fluorimeter and with a smartphone by analyzing the RGB components of the taken photographs. This sensory material is prepared with 99.75% of commercially available monomers and contains 0.25% of a sensory monomer based on a quinoline structure. The sensory motifs are chemically anchored to the polymeric structure, and, accordingly, no migration of organic substances from the material occurs during the sensing process. Our method has been tested with freshly prepared Zn(II) aqueous solutions, but also with biological samples from exudates of chronic wounds. The proposed methodology provides limits of detection (LOD) of 13 and 27 ppb when employing a water-soluble polymer (WsP) and a hydrophilic polymeric film (HP), respectively, using emission spectroscopy. The measurements have been contrasted with ICP-MS as the reference method, obtaining reliable data. This study is the starting point toward a larger investigation with patients, which will address the challenge of establishing a direct relationship between the concentration of zinc(II), other cations and also of amino acids, with the protease activity and, finally, with the state/evolution of chronic wounds. In this context, the proposed sensory material and others we are now working with will act as a simple and cheap method for this purpose.
PB Elsevier
SN 1381-5148
YR 2020
FD 2020-09
LK http://hdl.handle.net/10259/5396
UL http://hdl.handle.net/10259/5396
LA eng
NO FEDER (Fondo Europeo de Desarrollo Regional), and both the Spanish Ministerio de Economía, Industria y Competitividad (MAT2017-84501-R) and the Consejería de Educación—Junta de Castilla y León (BU061U16)
DS Repositorio Institucional de la Universidad de Burgos
RD 04-dic-2024