RT info:eu-repo/semantics/article
T1 Alcian blue pyridine variant interaction with DNA and RNA polynucleotides and G-quadruplexes: changes in the binding features for different biosubstrates
A1 Macii, Francesca
A1 Pérez Arnáiz, Cristina
A1 Arrico, Lorenzo
A1 Busto Vázquez, Natalia
A1 García Ruiz, Begoña
A1 Biver, Tarita
K1 Groove binding
K1 Partial intercalation
K1 Helix conformation
K1 Enthalpy-entropy compensation
K1 Anti-parallel
K1 Hybrid
K1 Química inorgánica
K1 Chemistry, Inorganic
K1 Bioquímica
K1 Biochemistry
AB This work concerns an analysis of the binding mechanism of a copper phthalocyanine (Alcian Blue-tetrakis(methylpyridinium) chloride, ABTP) to natural calf thymus DNA, G-quadruplexes (G4) and synthetic RNA polynucleotides in the form of double polyriboadenylic·polyribouridylic acid (poly(A)·poly(U)) or triple strands polyriboadenylic·2polyribouridylic acid (poly(A)·2poly(U)). ABTP is a well know dye that might undergo novel applications, but its interaction with DNA is scarcely studied and we lack information on possible RNA or G4 binding. This might be related to system complexity due to the presence of supramolecular dye-dye aggregates. Despite this, we show here that apparent parameters can be calculated, which provide information on the binding mechanism. Absorbance titrations in the presence of biosubstrate excess, melting and circular dichroism experiments show that ABTP binds to both RNAs and DNA. External/groove binding is the main feature for RNAs, whereas partial intercalation is the major binging mode for DNA. ABTP externally binds to both hybrid, parallel and anti-parallel G4s but seem to show a slightly different binding mode and a preference for anti-parallel structures. The thermodynamic features of the different systems are also discussed in the frame of the enthalpy-entropy compensation phenomenon.
PB Elsevier
SN 0162-0134
YR 2020
FD 2020-11
LK http://hdl.handle.net/10259/5475
UL http://hdl.handle.net/10259/5475
LA eng
NO la Caixa” Foundation (LCF/PR/PR12/11070003), Ministerio de Ciencia, Innovación y Universidades-FEDER (RTI2018- 102040-B-100) and Junta de Castilla y León-FEDER (BU305P18)
DS Repositorio Institucional de la Universidad de Burgos
RD 23-nov-2024