RT info:eu-repo/semantics/article T1 Formulation and preparation of water-In-oil-In-water emulsions loaded with a phenolic-rich Inner aqueous phase by application of high energy emulsification methods A1 Niknam, S. Mehdi A1 Escudero Barbero, Isabel A1 Benito Moreno, José Manuel K1 water-in-oil-in-water (W/O/W) emulsion K1 response surface methodology (RSM) K1 microfluidization K1 ultrasonic homogenization K1 rotor-stator mixing K1 stability analysis K1 Alimentos K1 Food K1 Ingeniería química K1 Chemical engineering AB Currently, industry is requesting proven techniques that allow the use of encapsulatedpolyphenols, rather than free molecules, to improve their stability and bioavailability. Response surfacemethodology (RSM) was applied in this work to determine the optimal composition and operatingconditions for preparation of water-in-oil-in-water (W/O/W) emulsions loaded with phenolic richinner aqueous phase from olive mill wastewater. A rotor-stator mixer, an ultrasonic homogenizerand a microfluidizer processor were tested in this study as high-energy emulsification methods.Optimum results were obtained by means of microfluidizer with 148 MPa and seven cycles inputlevels yielding droplets of 105.3 3.2 nm in average size and 0.233 0.020 of polydispersityindex. -potential, chemical and physical stability of the optimal W/O/W emulsion were alsoevaluated after storage. No droplet size growth or changes in stability and -potential were observed.Furthermore, a satisfactory level of phenolics retention (68.6%) and antioxidant activity (89.5%) after35 days of storage at room temperature makes it suitable for application in the food industry. PB MDPI YR 2020 FD 2020-10 LK http://hdl.handle.net/10259/5517 UL http://hdl.handle.net/10259/5517 LA eng NO Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) through grant number BU301P18, DS Repositorio Institucional de la Universidad de Burgos RD 23-nov-2024