RT info:eu-repo/semantics/article
T1 Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells
A1 Gerardi, Gisela
A1 Casali, Cecilia I.
A1 Cavia Saiz, Mónica
A1 Rivero Pérez, Maria Dolores
A1 Perazzo, Cecilia
A1 González San José, Mª Luisa
A1 Muñiz Rodríguez, Pilar
A1 Fernández Tome, María C.
K1 Food science
K1 Cell biology
K1 Plant products
K1 Cell differentiation
K1 Cytoskeleton
K1 Natural product
K1 Polyphenol
K1 Antioxidant
K1 Cancer research
K1 Renal system
K1 Wine pomace
K1 Polyphenols
K1 EMT
K1 Oxalate
K1 E-cadherin
K1 Alimentos
K1 Food
K1 Biología molecular
K1 Molecular biology
K1 Bioquímica
K1 Biochemistry
AB The functional renal epithelium is composed of differentiated and polarized tubular cells with a strong actin cortex and specialized cell-cell junctions. If, under pathological conditions, these cells have to resist higher kidney osmolarity, they need to activate diverse mechanisms to survive external nephrotoxic agents such as inflammation and oxidative stress. Wine pomace polyphenols exert protective effects on renal cells. In this study, two wine-pomace products and their protective effects upon promotion and preservation of normal cell differentiation and attenuation of oxalate-induced type II epithelial mesenchymal transition (EMT) are evaluated. Treatment with gastrointestinal and colonic bioavailable fractions from red (rWPP) and white (wWPP) wine pomaces, both in the presence and the absence of oxalate, showed similar cell numbers and nuclear size than the non-treated differentiated MDCK cells. Immunofluorescence analysis showed the reduction of morphological changes and the preservation of cellular junctions for the rWPP and wWPP pre-treatment of cells exposed to oxalate injury. Hence, both rWPP and wWPP attenuated oxalate type II EMT in MDCK cells that conserved their epithelial morphology and cellular junctions through the antioxidant activities of grape pomace polyphenols.
PB Elsevier
SN 2405-8440
YR 2020
FD 2020-11
LK http://hdl.handle.net/10259/5577
UL http://hdl.handle.net/10259/5577
LA eng
NO Agencia Nacional de Promoci on Científica y Tecnol ogica (PICT 2016-1055), the University of Buenos Aires (UBACYT 2014-2017, 20020130100658BA) and the Ministerio de Ciencia, Innovaci on y Universidades (PGC2018-097113B100).
DS Repositorio Institucional de la Universidad de Burgos
RD 25-dic-2024