RT info:eu-repo/semantics/article
T1 Assessment of Physico-Chemical and Toxicological Properties of Commercial 2D Boron Nitride Nanopowder and Nanoplatelets
A1 Domi, Brixhilda
A1 Bhorkar, Kapil
A1 Rumbo Lorenzo, Carlos
A1 Sygellou, Labrini
A1 Yannopoulos, Spyros N.
A1 Barros García, Rocío
A1 Quesada Pato, Roberto
A1 Tamayo Ramos, Juan Antonio
K1 2D boron nitride
K1 eukaryotic model
K1 nanotoxicity
K1 cell viability
K1 oxidative stress
K1 Materia-Estructura
K1 Matter-Constitution
K1 Materiales
K1 Materials
AB Boron nitride (BN) nanomaterials have been increasingly explored for potential applications in chemistry and biology fields (e.g., biomedical, pharmaceutical, and energy industries) due to their unique physico-chemical properties. However, their safe utilization requires a profound knowledge on their potential toxicological and environmental impact. To date, BN nanoparticles have been considered to have a high biocompatibility degree, but in some cases, contradictory results on their potential toxicity have been reported. Therefore, in the present study, we assessed two commercial 2D BN samples, namely BN-nanopowder (BN-PW) and BN-nanoplatelet (BN-PL), with the objective to identify whether distinct physico-chemical features may have an influence on the biological responses of exposed cellular models. Morphological, structural, and composition analyses showed that the most remarkable difference between both commercial samples was the diameter of their disk-like shape, which was of 200–300 nm for BN-PL and 100–150 nm for BN-PW. Their potential toxicity was investigated using adenocarcinomic human alveolar basal epithelial cells (A549 cells) and the unicellular fungus Saccharomycescerevisiae, as human and environmental eukaryotic models respectively, employing in vitro assays. In both cases, cellular viability assays and reactive oxygen species (ROS) determinations where performed. The impact of the selected nanomaterials in the viability of both unicellular models was very low, with only a slight reduction of S. cerevisiae colony forming units being observed after a long exposure period (24 h) to high concentrations (800 mg/L) of both nanomaterials. Similarly, BN-PW and BN-PL showed a low capacity to induce the formation of reactive oxygen species in the studied conditions. Even at the highest concentration and exposure times, no major cytotoxicity indicators were observed in human cells and yeast. The results obtained in the present study provide novel insights into the safety of 2D BN nanomaterials, indicating no significant differences in the toxicological potential of similar commercial products with a distinct lateral size, which showed to be safe products in the concentrations and exposure conditions tested.
PB MDPI
YR 2021
FD 2021-01
LK http://hdl.handle.net/10259/5588
UL http://hdl.handle.net/10259/5588
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 25-abr-2024