RT info:eu-repo/semantics/article
T1 Aspergillus niger citrate exporter revealed by comparison of two alternative citrate producing conditions
A1 Odoni, Dorett I
A1 Vazquez Vilar, Marta
A1 Gaal, Merlijn P van
A1 Schonewille, Tom
A1 Santos, Vitor AP Martins dos
A1 Tamayo Ramos, Juan Antonio
A1 Suarez Diez, Maria
A1 Schaap, Peter J.
K1 Aspergillus niger
K1 citrate
K1 transport
K1 transcriptomics
K1 homology
K1 MDR
K1 Microbiología
K1 Microbiology
AB Currently, there is no consensus regarding the mechanism underlying Aspergillus niger citrate biosynthesis and secretion. We hypothesise that depending on the experimental setup, extracellular citrate accumulation can have fundamentally different underlying transcriptomic landscapes. We show that varying the amount and type of supplement of an arginine auxotrophic A. niger strain results in transcriptional down-regulation of citrate metabolising enzymes in the condition in which more citrate is accumulated extracellularly. This contrasts with the transcriptional adaptations when increased citrate production is triggered by iron limitation. By combining gene expression data obtained from these two very distinct experimental setups with hidden Markov models and transporter homology approaches, we were able to compile a shortlist of the most likely citrate transporter candidates. Two candidates (An17g01710 and An09g06720m.01) were heterologously expressed in the yeast Saccharomyces cerevisiae, and one of the resultant mutants showed the ability to secrete citrate. Our findings provide steps in untangling the complex interplay of different mechanisms underlying A. niger citrate accumulation, and we demonstrate how a comparative transcriptomics approach complemented with further bioinformatics analyses can be used to pinpoint a fungal citrate exporter.
PB Oxford University Press
SN 0378-1097
YR 2019
FD 2019-04
LK http://hdl.handle.net/10259/5589
UL http://hdl.handle.net/10259/5589
LA eng
NO grant in the framework of the BE-BASIC program F01.011 Transport processes in the production of organic acids by Aspergillus niger, and the WUR IPOP Systems Biology program KB-17-003.02.026 Genome-wide metabolic modelling and data integration of organic acid production in filamentous fungi.
DS Repositorio Institucional de la Universidad de Burgos
RD 20-abr-2024