RT info:eu-repo/semantics/article T1 Subcritical water as hydrolytic medium to recover and fractionate the protein fraction and phenolic compounds from craft brewer’s spent grain A1 Alonso Riaño, Patricia A1 Sanz Díez, Mª Teresa A1 Benito Román, Oscar A1 Beltrán Calvo, Sagrario A1 Trigueros Andrés, Ester K1 Subcritical water extraction K1 Biorefinery K1 Breweŕs spent grain K1 Protein K1 Amino acids K1 Individual phenolic compounds K1 Antioxidant capacity K1 Ingeniería química K1 Chemical engineering K1 Alimentos K1 Food AB The valorization of the brewer’s spent grain (BSG) generated in a craft beer industry was studied by subcritical water hydrolysis in a semi-continuous fixed-bed reactor. Temperature was varied from 125 to 185 °C at a constant flow rate of 4 mL/min. Biomass hydrolysis yielded a maximum of 78% of solubilized protein at 185 °C. Free amino acids presented a maximum level at 160 °C with a value of 55 mg free amino acids/gprotein-BSG. Polar amino acid presented a maximum at lower temperatures than non-polar amino acids. The maximum in total phenolic compounds was reached at 185 °C. This maximum is the same for aldehyde phenolic compounds such as vanillin, syringic and protocatechuic aldehyde; however, for hydroxycinnamic acids, such as ferulic acid and p-coumaric, the maximum was obtained at 160 °C. This allows a fractionation of the bioactive compounds. Subcritical water addresses opportunities for small breweries to be incorporated within the biorefinery concept. PB Elsevier SN 0308-8146 YR 2021 FD 2021-07 LK http://hdl.handle.net/10259/5672 UL http://hdl.handle.net/10259/5672 LA eng NO Agencia Estatal de Investigación [grant number PID2019-104950RB-I00 / AEI / 10.13039/501100011033] and the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [grant numbers BU301P18 and BU050P20] DS Repositorio Institucional de la Universidad de Burgos RD 20-abr-2024