RT info:eu-repo/semantics/article T1 Polymer film as starch azure container for the easy diastase activity determination in honey A1 González Ceballos, Lara A1 Fernández Muiño, Miguel Ángel A1 Osés Gómez, Sandra María A1 Sancho Ortiz, María Teresa A1 Ibeas Cortes, Saturnino A1 Reglero Ruiz, José Antonio A1 Vallejos Calzada, Saúl K1 Honey K1 Diastase activity K1 Starch azure K1 Polymer K1 Film K1 Química orgánica K1 Chemistry, Organic K1 Química física K1 Chemistry, Physical and theoretical K1 Alimentos K1 Food AB A new original application for a polyacrylic film based on the monomers 2-(dimethylamino)ethyl methacrylate (NNDA), 2-hydroxyethyl acrylate (2HEA) and methylmethacrylate (MMA) as a starch azure container has been set up for a simple determination of honey diastase activity. The proposed method is based on the correlation of reducing sugars generated during the enzymatic process with the Schade reference assay. The polyacrylic film is charged with starch azure acting as a container for this substance; thus, the starch does not interfere in the measurement of reducing sugars, so that the diastase activity is easily calculated. The method has been contrasted with Schade method, showing good correlation and differences under 0.4% between methods in some honey samples. The polyacrylic film has great potential for the routine honey diastase activity assessment in small laboratories, dramatically reducing analysis time and cost. PB Elsevier SN 0308-8146 YR 2021 FD 2021-09 LK http://hdl.handle.net/10259/5676 UL http://hdl.handle.net/10259/5676 LA eng NO FEDER (Fondo Europeo de Desarrollo Regional), and both the Spanish Ministerio de Economía, Industria y Competitividad (MAT2017-84501-R) and the Consejería de Educación—Junta de Castilla y León (BU306P18 and BU041G18) DS Repositorio Institucional de la Universidad de Burgos RD 22-dic-2024