RT info:eu-repo/semantics/article
T1 Global transcriptional response of aspergillus niger to blocked active citrate export through deletion of the exporter gene
A1 Laothanachareon, Thanaporm
A1 Bruinsma, Lyon
A1 Nijsse, Bart
A1 Schonewille, Tom
A1 Suarez Diez, Maria
A1 Tamayo Ramos, Juan Antonio
A1 Santos, Vitor AP Martins dos
A1 Schaap, Peter J.
K1 Aspergillus niger
K1 citrate exporter
K1 citT gene
K1 cexA gene
K1 transcriptome
K1 citric acid
K1 oxalic acid
K1 Microbiología
K1 Microbiology
AB Aspergillus niger is the major industrial citrate producer worldwide. Export as well asuptake of citric acid are believed to occur by active, proton-dependent, symport systems. Bothare major bottlenecks for industrial citrate production. Therefore, we assessed the consequencesof deleting the citT gene encoding the A. niger citrate exporter, effectively blocking active citrateexport. We followed the consumption of glucose and citrate as carbon sources, monitored thesecretion of organic acids and carried out a thorough transcriptome pathway enrichment analysis.Under controlled cultivation conditions that normally promote citrate secretion, the knock-out strainsecreted negligible amounts of citrate. Blocking active citrate export in this way led to a reducedglucose uptake and a reduced expression of high-affinity glucose transporter genes, mstG and mstH.The glyoxylate shunt was strongly activated and an increased expression of the OAH gene wasobserved, resulting in a more than two-fold higher concentration of oxalate in the medium. Deletionof citT did not affect citrate uptake suggesting that citrate export and citrate uptake are uncoupledfrom the system.
PB MDPI
YR 2021
FD 2021-06
LK http://hdl.handle.net/10259/5778
UL http://hdl.handle.net/10259/5778
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 25-abr-2024