RT info:eu-repo/semantics/article
T1 Kinky DNA in solution: Small-angle-scattering study of a nucleosome positioning sequence
A1 Schindler, Torben
A1 González, Adrián
A1 Boopathi, Ramachandran
A1 Marty Roda, Marta
A1 Romero Santacreu, Lorena
A1 Wildes, Andew R.
A1 Porcar, Lionel
A1 Martel, Anne
A1 Theodorakopoulos, Nikos
A1 Cuesta López, Santiago
A1 Angelov, Dimitar
A1 Unruh, Tobias
A1 Peyrard, Michel
K1 Física
K1 Physics
AB DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-acceptedpersistence length of lp ≈ 500Å is inconsistent with recent studies on short-chain DNA that show much greaterflexibility but do not probe its origin. We have performed x-ray and neutron small-angle scattering on a shortDNA sequence containing a strong nucleosome positioning element and analyzed the results using a modifiedKratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klugin 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Ourconclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead toincreased flexibility and by an analysis of data from another sequence, which does not have kinks, but where ourmethod can detect a locally enhanced flexibility due to an AT domain.
PB American Physical Society
SN 2470-0045
YR 2018
FD 2018-10
LK http://hdl.handle.net/10259/5784
UL http://hdl.handle.net/10259/5784
LA eng
NO Spanish Ministry of Economy, Industry and Competitiveness (BES-2013-065453, EEBB-I-2015-09973, FIS2012-38827). S.C.L. and UC-154 are grateful for the support of Junta de Castilla y Leon (Spain) Nanofibersafe BU079U16. D.A. acknowledges funding from the Agence Nationale de la Recherche through ANR-12-BSV5-0017-01 “Chrome” and ANR-17-CE11-0019-03 “Chrom3D” grants. N.T. acknowledges support by the project Advanced Materials and Devices (MIS 5002409, Competitiveness, Entrepreneurship and Innovation, NSRF 2014-2020) cofinanced by Greece and the European Regional Development Fund.
DS Repositorio Institucional de la Universidad de Burgos
RD 21-nov-2024