RT info:eu-repo/semantics/article
T1 Dopamine-functionalized graphene oxide as a high-performance material for biosensing
A1 Vulcano, Fabio
A1 Kovtun, Alessandro
A1 Bettini, Cristian
A1 Xia, Zhenyuan
A1 Liscio, Andrea
A1 Terzi, Fabio
A1 Heras Vidaurre, Aránzazu
A1 Colina Santamaría, Álvaro
A1 Zanfrognini, Barbara
A1 Melucci, Manuela
K1 graphene oxide
K1 spectroelectrochemistry
K1 NADH
K1 chemical functionalization
K1 1,2-dihydroxyphenyl moieties
K1 Química analítica
K1 Chemistry, Analytic
AB We describe a nanocomposite material for the electrochemical detection of β-nicotinamide adenine dinucleotide (NADH), a coenzyme involved in redox reactions of all living cells and in the detection of many organic species by electrochemical biosensors. The composite is made of nanosheets of electrochemically exfoliated graphene oxide (EGO) covalently functionalized with dopamine (DP) molecules. The EGODP material finally obtained is rich of 1,2-dihydroxyphenyl moieties and is able to detect NADH at a particular low potential value with higher sensitivity with respect to pristine EGO.To study the effectiveness of 1,2-dihydroxyphenyl moieties in inducing electrocatalytic oxidation of NADH, we combined standard voltammetric techniques with UV–Vis absorption spectroelectrochemistry, which allowed us to measure the variations in composition occurring at the electrode|solution interface, i.e. to measure the consumption rate of NADH.Spectroelectrochemical tests performed by polarising the electrode at a fixed potential value were finally used to compare the performance of EGODP with both EGO and EGO-DP blend (MIX) for the detection of NADH. The covalently functionalized EGO (EGODP) shows sensitivity to NADH up to 300 M−1, around 180 % and 140 % better than either pristine EGO or MIX, respectively.
PB IOP Publishing
SN 2053-1583
YR 2020
FD 2020-04
LK http://hdl.handle.net/10259/6119
UL http://hdl.handle.net/10259/6119
LA spa
NO Spanish Ministerio de Economía y Competitividad (Grants CTQ2017-83935-R-AEI/FEDERUE), Ministerio de Ciencia, Innovación y Universidades (RED2018-102412-T) and Junta de Castilla y León (Grant BU297P18).
DS Repositorio Institucional de la Universidad de Burgos
RD 24-nov-2024