RT info:eu-repo/semantics/article
T1 Derivative UV/Vis spectroelectrochemistry in a thin-layer regime: deconvolution and simultaneous quantification of ascorbic acid, dopamine and uric acid
A1 Olmo Alonso, Fabiola
A1 Garoz Ruiz, Jesús
A1 Colina Santamaría, Álvaro
A1 Heras Vidaurre, Aránzazu
K1 Spectroelectrochemistry
K1 PARAFAC
K1 Quantitative analysis
K1 Derivative of absorbance with respect towavelength
K1 Química analítica
K1 Chemistry, Analytic
AB In this work, UV/Vis spectroelectrochemistry (SEC), in a thin-layer regime and parallel configuration, is selected to solve acomplex mixture that contains dopamine (DA), ascorbic acid (AA) and uric acid (UA). These molecules, like many otherbiological compounds, are assuming a highly important place in analytical and biomedical fields due to the fundamental rolethat they play in human metabolism. In addition, low or high levels of these compounds are associated with diseases such asParkinson’s disease. For this reason, the quantification of these biomolecules is becoming increasingly critical. However, somedrawbacks must be overcome, because the three molecules coexist in the human body, and the species are subject to mutualinterference. In fact, they are all oxidized at similar potentials, and their UV/Vis absorption bands overlap, greatly complicatingtheir quantification. For this reason, derivative SEC together with suitable chemometric tools such as PARAFAC are proposed tosolve this complex matrix. This technique allows us to separate the contribution of each of these molecules present in a sampleand to quantify all of them, achieving high resolution and reproducibility. Besides, detection limits at the micromolar level areachieved for DA, AA and UA in mixture solutions. This work thus demonstrates the great potential for derivative potentiodynamicSEC combined with the appropriate chemometric tools in solving complex mixtures, a field where SEC is still taking thefirst steps.
PB Springer
SN 1618-2642
YR 2020
FD 2020-09
LK http://hdl.handle.net/10259/6124
UL http://hdl.handle.net/10259/6124
LA spa
NO Ministerio de Economía y Competitividad (Grants CTQ2017-83935-RAEI/FEDER, UE), Junta de Castilla y León (Grant BU297P18) andMinisterio de Ciencia, Innovación y Universidades (RED2018-102412-T). F.O. is grateful for the contract funded by Junta de Castilla y León, theEuropean Social Fund and the Youth Employment Initiative. J.G.R.thanks theMinisterio de Economía y Competitividad for his postdoctoralcontract (CTQ2017-83935-R AEI/FEDER, UE).
DS Repositorio Institucional de la Universidad de Burgos
RD 11-dic-2024