RT info:eu-repo/semantics/article
T1 Multiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometer
A1 Prieto Santiago, Virginia
A1 Cavia Camarero, María del Mar
A1 Barba, Francisco J.
A1 Alonso de la Torre, Sara
A1 Carrillo Pérez, Celia
K1 Triple quadrupole
K1 Multiple reaction monitoring
K1 Oligosaccharides
K1 Legumes
K1 Biología molecular
K1 Molecular biology
K1 Alimentos
K1 Food
AB Raffinose family oligosaccharides are non-digestible compounds considered as dietary prebiotics with health-related properties. Hence, it is important to develop highly specific methods for their determination. An analytical method is developed in this study for oligosaccharide identification and quantification using liquid chromatography-tandem mass spectrometry equipped with a triple quadrupole analyser operating in Multiple Reaction Monitoring mode. Raffinose, stachyose and verbascose are separated in a 10-minute run and the method is validated over a broad concentration range, showing good linearity, accuracy, precision and high sensitivity. A low-cost, short eco-friendly procedure for oligosaccharide extraction from legumes, with a high recovery rate extraction, good repeatability and reproducibility is also proposed. No plant-matrix effects were demonstrated. The method applied to the screening of 28 different legumes revealed species-related traits for oligosaccharide distribution, highlighting Pisum sativum (9.22 g/100 g) as the richest source of these prebiotics and its suitability as a functional food ingredient.
PB Elsevier
SN 0308-8146
YR 2022
FD 2022-01
LK http://hdl.handle.net/10259/6196
UL http://hdl.handle.net/10259/6196
LA eng
NO Prieto-Santiago is a research assistant funded by Junta de Castilla y León (Spain) (Grant No. UBU-10-B).
DS Repositorio Institucional de la Universidad de Burgos
RD 27-abr-2024