RT info:eu-repo/semantics/article T1 Synthesis and oxidative stability of monoacylglycerols containing polyunsaturated fatty acids by enzymatic glycerolysis in a solvent-free system A1 Palacios Santamaría, David A1 Busto Núñez, Mª Dolores A1 Albillos García, Silvia María A1 Ortega Santamaría, Natividad K1 Desirability function K1 Lipase-catalyzed glycerolysis K1 Monoacylglycerols K1 Oxidative stability K1 Polyunsaturated fatty acids K1 Bioquímica K1 Biochemistry K1 Biología molecular K1 Molecular biology AB Monoacylglycerols (MAG) containing polyunsaturated fatty acids (PUFA) have been synthesized by glycerolysis of anchovy oil using Lipozyme RM-IM in a solvent-free system. The experimental conditions, reaction temperature, substrate molar ratio (glycerol/triacylglycerol, Gly/TAG) and enzyme concentration were studied. A response surface methodology was employed to analyze the effect of the individual variables on MAG production and oxidative stability with respect to induction time. The operating conditions that simultaneously optimized MAG yields and oxidative stability were a reaction temperature of 40 °C, a Gly/TAG molar ratio of 2:1 and 6% enzyme load. The yield obtained was 20.34% of MAG (with 12.12% of 2-MAG) and an induction time of 1.88 h. PB Elsevier SN 0023-6438 YR 2022 FD 2022-01 LK http://hdl.handle.net/10259/6197 UL http://hdl.handle.net/10259/6197 LA eng DS Repositorio Institucional de la Universidad de Burgos RD 29-mar-2024