RT info:eu-repo/semantics/article
T1 Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer
A1 González Ceballos, Lara
A1 Guirado Moreno, José Carlos
A1 Utzeri, Gianluca
A1 García Pérez, José Miguel
A1 Fernández Muiño, Miguel Ángel
A1 Osés Gómez, Sandra María
A1 Sancho Ortiz, María Teresa
A1 Arnáiz Alonso, Ana
A1 Valente, Artur J. M.
A1 Vallejos Calzada, Saúl
K1 Catechol
K1 1,2-Dihydroxybenzene
K1 Phenylboronic acid
K1 Glucose oxidase
K1 Catalase
K1 Honey
K1 Química orgánica
K1 Chemistry, Organic
K1 Química física
K1 Chemistry, Physical and theoretical
K1 Alimentos
K1 Food
AB Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample).
PB Elsevier
SN 0308-8146
YR 2023
FD 2023-03
LK http://hdl.handle.net/10259/7141
UL http://hdl.handle.net/10259/7141
LA eng
NO We gratefully acknowledge the financial support provided by all funders. Author Jose Miguel García received grant PID2020-113264RB-I00 / AEI / 10.13039/501100011033 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. Ana Arnaiz received funding from Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). We also gratefully acknowledge European Regional Development Fund (ERDF). Gianluca Utzeri thanks Fundação para a Ciência e a Tecnologia (FCT, Portugal) for PhD grant (SFR/BD/146358/2019). The Coimbra Chemistry Centre is supported by the FCT, through Projects UIDB/00313/2020 and UIDP/00313/2020. To all the beekeepers who provided a sample of honey for this study.
DS Repositorio Institucional de la Universidad de Burgos
RD 23-nov-2024