RT info:eu-repo/semantics/article
T1 Lab-on-a-chip for the easy and visual detection of SARS-CoV-2 in saliva based on sensory polymers
A1 Arnáiz Alonso, Ana
A1 Guirado Moreno, José Carlos
A1 Guembe García, Marta
A1 Barros García, Rocío
A1 Tamayo Ramos, Juan Antonio
A1 Fernández Pampín, Natalia
A1 García Pérez, José Miguel
A1 Vallejos Calzada, Saúl
K1 Peptide
K1 Substrate
K1 Fluorimetry
K1 Immobilization
K1 COVID-19
K1 Paper-supported
K1 Coating
K1 Pandemics
K1 Química orgánica
K1 Chemistry, Organic
K1 Enfermedades infecciosas
K1 Communicable diseases
AB The initial stages of the pandemic caused by SARS-CoV-2 showed that early detection of the virus in a simple way is the best tool until the development of vaccines. Many different tests are invasive or need the patient to cough up or even drag a sample of mucus from the throat area. Besides, the manufacturing time has proven insufficient in pandemic conditions since they were out of stock in many countries. Here we show a new method of manufacturing virus sensors and a proof of concept with SARS-CoV-2. We found that a fluorogenic peptide substrate of the main protease of the virus (Mpro) can be covalently immobilized in a polymer, with which a cellulose-based material can be coated. These sensory labels fluoresce with a single saliva sample of a positive COVID-19 patient. The results matched with that of the antigen tests in 22 of 26 studied cases (85% success rate).
PB Elsevier
SN 0925-4005
YR 2023
FD 2023-03
LK http://hdl.handle.net/10259/7316
UL http://hdl.handle.net/10259/7316
LA eng
NO We gratefully acknowledge the financial support provided by all funders. Author Saul Vallejos coordinates the project leading to these results, which has received funding from "La Caixa" Foundation, under agreement LCF/PR/PR18/51130007. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author Jose Miguel García received grant PID2020–113264RB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. Ana Arnaiz received funding from Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). Finally, all the authors want to thank the support provided by City Hall of Villadiego “Ayuntamiento de Villadiego” when looking for participants for the proof of concept.
DS Repositorio Institucional de la Universidad de Burgos
RD 24-nov-2024