RT info:eu-repo/semantics/article
T1 Filtration of subcritical water hydrolysates from red macroalgae byproducts with ultraporous ceramic membranes for oligosaccharide and peptide fractionation
A1 Trigueros Andrés, Ester
A1 Sanz Díez, Mª Teresa
A1 Beltrán Calvo, Sagrario
A1 Ruiz Pérez, María Olga
K1 Peptide purification
K1 Oligosaccharide recovery
K1 Membrane fouling
K1 Macroalga byproducts
K1 Biorefinery
K1 Química orgánica
K1 Chemistry, Organic
K1 Ingeniería química
K1 Chemical engineering
AB An ultrafiltration-based process for oligosaccharide and peptide fractionation from a macroalgae subcriticalwater hydrolysate was studied. A wide range of separation results was obtained depending on the membranepore. 100 kDa cut-off size was enough for hydrolysate clarification with total retention of colloidal materials.Oligosaccharides present in the hydrolysate showed the highest retention with all membranes, glucans mostly,followed by galactans, and finally arabinans. Peptides obtained after subcritical water treatment were some ofthe lowest rejected compounds, even using a 5 kDa membrane. The increase in temperature from 20 to 50 ◦C andfeed flow rate from 6.6 to 11.2 L/h enhanced permeate flux for 5 kDa membrane, without perturbing themembrane retention. The Hermia’s models identified the cake layer resistance as the major fouling resistance inhydrolysate filtrations at 20 ◦C, but standard pore blockage was the principal fouling mechanism at 50 ◦C. Afractionation process with sequential filtration stages at 20 ◦C and TMP = 1.1 bar was examined. Oligosaccharides were fractionated in the retentates of the sequential filtrations with 100, 5 and 1 kDa membranes. Thefinal permeate collected from the 1 kDa membrane was freeze-dried to obtain a peptide-rich solid (71 wt%) thatcould be used in different applications.
PB Elsevier
SN 0376-7388
YR 2022
FD 2022-10
LK http://hdl.handle.net/10259/7453
UL http://hdl.handle.net/10259/7453
LA eng
NO The authors gratefully acknowledge the financial support provided by the Agencia Estatal de Investigación [grant number PID2019–104950RB-I00/AEI/10.13039/501100011033] and by the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERF) [grant number BU050P20]. E. Trigueros predoctoral contract was funded by the JCyL and the European Social Fund (ESF) by ORDEN EDU/574/2018.
DS Repositorio Institucional de la Universidad de Burgos
RD 09-may-2024