RT info:eu-repo/semantics/article T1 Valorization of brewer’s spent grain by consecutive supercritical carbon dioxide extraction and enzymatic hydrolysis A1 Alonso Riaño, Patricia A1 Melgosa Gómez, Rodrigo A1 Trigueros Andrés, Ester A1 Illera Gigante, Alba Ester A1 Beltrán Calvo, Sagrario A1 Sanz Díez, Mª Teresa K1 Biorefinery K1 Brewer's spent grain K1 Supercritical fluid extraction K1 Enzymatic hydrolysis K1 Biología K1 Biology K1 Ingeniería química K1 Chemical engineering AB The double effect of supercritical carbon dioxide, sc-CO2, in a biorefinery concept applied to brewer’s spent grain(BSG) was assessed in this work. Extraction conditions to remove and valorize the lipophilic fraction werestudied (20–40 MPa and 40–80 ◦C) obtaining a maximum yield of 5.70 ± 0.07 g/100 gBSG at 80 ◦C and 40 MPa.High pressures and temperatures resulted in higher content of total phenolic and flavonoid compounds, as well ashigher antioxidant capacity. It was observed an improvement of the enzymatic hydrolysis yield by cellulase inthe sc-CO2 treated BSG compared to the non-treated. This improvement could be partially attributed to theremoval of the lipid fraction and to morphological changes of BSG after sc-CO2. Based on this double benefit, scCO2 can play an important role on biomass valorization. PB Elsevier SN 0308-8146 YR 2022 FD 2022-12 LK http://hdl.handle.net/10259/7470 UL http://hdl.handle.net/10259/7470 LA eng NO This work was supported by the Agencia Estatal de Investigación [grant number PID2019-104950RB-I00 / AEI / https://doi.org/10.130 39/501100011033] and the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [grant numbers BU301P18 and BU050P20]. E. Trigueros and P. Alonso-Riano ˜ predoctoral contracts were funded by JCyL and the European Social Fund (ESF) by ORDEN EDU/574/2018, de 28 de mayo and ORDEN EDU/556/2019, de 5 de junio, respectively. DS Repositorio Institucional de la Universidad de Burgos RD 23-nov-2024