RT info:eu-repo/semantics/article
T1 Comparative toxicological assessment of three soils polluted with different levels of hydrocarbons and heavy metals using in vitro and in vivo approaches
A1 Parra de la Parra, Sandra de la
A1 González, Verónica
A1 Solórzano Vives, Patricia
A1 Curiel Alegre, Sandra
A1 Velasco Arroyo, Blanca
A1 Rad Moradillo, Juan Carlos
A1 Barros García, Rocío
A1 Tamayo Ramos, Juan Antonio
A1 Rumbo Lorenzo, Carlos
K1 HepG2 cells
K1 Saccharomyces cerevisiae
K1 Pseudomonas putida
K1 Enchytraeus crypticus
K1 Soil contamination
K1 Química analítica
K1 Chemistry, Analytic
AB The biological effects induced by the pollutants present in soils, together with the chemical and physical characterizations, are good indicators to provide a general overview of their quality. However, the existence ofstudies where the toxicity associated to soils contaminated with mixtures of pollutants applying both in vitro andin vivo models are scarce. In this work, three soils (namely, Soil 001, Soil 002 and Soil 013) polluted withdifferent concentrations of hydrocarbons and heavy metals were evaluated using different organisms representative of human (HepG2 human cell line) and environmental exposure (the yeast Saccharomyces cerevisiae, theGram-negative bacterium Pseudomonas putida and, for the in vivo evaluation, the annelid Enchytraeus crypticus). Invitro assays showed that the soluble fraction of the Soil 001, which presented the highest levels of heavy metals,represented a great impact in the viability of the HepG2 cells and S. cerevisiae, while organic extracts from Soils002 and 013 caused a slight decrease in the viability of HepG2 cells. In addition, in vivo experiments showed thatSoils 001 and 013 affected the survival and the reproduction of E. crypticus. Altogether, these results provide ageneral overview of the potential hazards associated to three specific contaminated sites in a variety of organisms, showing how different concentrations of similar pollutants affect them, and highlights the relevance oftesting both organic and soluble extracts when in vitro safety assays of soils are performed.
PB Elsevier
SN 0269-7491
YR 2022
FD 2022-12
LK http://hdl.handle.net/10259/7490
UL http://hdl.handle.net/10259/7490
LA eng
NO This work received funding from the GREENER project of the European Union’s Horizon 2020 research and innovation program (Grant Agreement No. 826312). S. Curiel-Alegre was granted with a predoctoral contract by Junta de Castilla y León and the European Social Fund (ORDEN EDU/1508/2020, de 15 de diciembre). We thank Mireya Pedrero and Andrea Martínez for their technical support. We also thank Institute of Technology Carlow and Shandong Academy of Sciences for kindly providing us with soil samples.
DS Repositorio Institucional de la Universidad de Burgos
RD 05-may-2024