RT info:eu-repo/semantics/article
T1 Characterization of Planktochlorella nurekis Extracts and Virucidal Activity against a Coronavirus Model, the Murine Coronavirus 3
A1 Graff Reis, Jacqueline
A1 Dai Prá, Isabella
A1 Michelon, William
A1 Viancelli, Aline
A1 Piedrahita Marquez, David Guillermo
A1 Schmitz, Caroline
A1 Maraschin, Marcelo
A1 Moura, Sidnei
A1 Thaís Silva, Izabella
A1 Oliveira Costa, Geovanna de
A1 Tizziani, Tiago
A1 Sandjo, Louis P.
A1 Rodríguez Lázaro, David
A1 Fongaro, Gislaine
K1 Coronavirus
K1 SARS-CoV-2
K1 Murine coronavirus
K1 Metabolomic approach
K1 NMR biomarkers
K1 UPLC-MS
K1 Viricidal activity
K1 Antiviral activity
K1 Microbiología
K1 Microbiology
AB Certain members of the Coronaviridae family have emerged as zoonotic agents andhave recently caused severe respiratory diseases in humans and animals, such as SARS, MERS,and, more recently, COVID-19. Antivirals (drugs and antiseptics) capable of controlling viruses atthe site of infection are scarce. Microalgae from the Chlorellaceae family are sources of bioactivecompounds with antioxidant, antiviral, and antitumor activity. In the present study, we aimed toevaluate various extracts from Planktochlorella nurekis in vitro against murine coronavirus-3 (MHV-3),which is an essential human coronavirus surrogate for laboratory assays. Methanol, hexane, anddichloromethane extracts of P. nurekis were tested in cells infected with MHV-3, and characterizedby UV-vis spectrophotometry, nuclear magnetic resonance (NMR) spectroscopy, ultraperformanceliquid chromatography-mass spectrometry (UPLC-MS), and the application of chemometrics throughprincipal component analysis (PCA). All the extracts were highly efficient against MHV-3 (more thana 6 Log unit reduction), regardless of the solvent used or the concentration of the extract, but thedichloromethane extract was the most effective. Chemical characterization by spectrophotometryand NMR, with the aid of statistical analysis, showed that polyphenols, carbohydrates, and isoprenederivatives, such as terpenes and carotenoids have a more significant impact on the virucidal potential.Compounds identified by UPLC-MS were mainly lipids and only found in the dichloromethaneextract. These results open new biotechnological possibilities to explore the biomass of P. nurekis; it isa natural extract and shows low cytotoxicity and an excellent antiviral effect, with low productioncosts, highlighting a promising potential for development and implementation of therapies againstcoronaviruses, such as SARS-CoV-2.
PB MDPI
YR 2022
FD 2022-11
LK http://hdl.handle.net/10259/7507
UL http://hdl.handle.net/10259/7507
LA eng
NO This research was funded LVA-MIP-CCB-UFSC/Sigpex: 201917940, and CNPq, CAPES-DS.
DS Repositorio Institucional de la Universidad de Burgos
RD 29-abr-2024