RT info:eu-repo/semantics/article T1 Subcritical Water as Pretreatment Technique for Bioethanol Production from Brewer’s Spent Grain within a Biorefinery Concept A1 Alonso Riaño, Patricia A1 Amândio, Mariana S.T. A1 Xavier, Ana María Rebelo Barreto A1 Beltrán Calvo, Sagrario A1 Sanz Díez, Mª Teresa K1 Subcritical water pretreatment K1 Brewer's spent grain K1 Lignocellulosic biomass K1 High solids' loading K1 Simultaneous saccharification and fermentation K1 Separate hydrolysis and fermentation K1 Ingeniería química K1 Chemical engineering AB Bioeconomy and environmental issues envisage industrial by-products such as Brewer’sspent grain (BSG) as renewable resources for their recycling and reuse within a biorefinery concept.This study aimed to investigate the production of bioethanol from subcritical water (subW) pretreatedBSG, following the conversion of the BSG biopolymers cellulose and hemicelluloses. The subWpretreatment was performed in a batch reactor at 174 ◦C, during 60 min and 5% (w/v) of dry BSGcharge. The behavior of BSG biopolymers under subW pretreatment was monitored by evaluatingthe chemical composition of the liquid and solid streams and the chemical and structural changescaused in the solid residues by scanning electron microscope (SEM), CHNS elemental analysisand water retention value (WRV). The production of bioethanol from subW-pretreated BSG wasassessed by separate hydrolysis and fermentation (SHF) and also by simultaneous saccharificationand fermentation (SSF) by using the enzymatic cocktail Celluclast 1.5 L (40 FPU/gsolids) and the yeastEthanol Red®. The higher bioethanol productivity (1.073 g·L−1·h−1) and concentration (32.18 g/L)were achieved by SSF with higher solids’ loading (25%) and following a fed-batch strategy. Theseresults suggest that subcritical water pretreatment is a promising technology for the valorization ofBSG as a feedstock for second-generation bioethanol production. PB MDPI YR 2022 FD 2022-11 LK http://hdl.handle.net/10259/7526 UL http://hdl.handle.net/10259/7526 LA eng NO This work was supported by the Agencia Estatal de Investigación (ES) [grant numbers PID2019–104950RB-I00/AEI/10.13039/501100011033, TED2021–129311B-I00 and PDC2022–133443-I00] and the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [grant number BU050P20]. The P. Alonso-Riaño predoctoral contract was funded by JCyL and the European Social Fund (ESF) by ORDEN EDU/556/2019, de 5 de junio. This work was also developed within the scope of the project CICECO-Aveiro Institute of Materials (PT), UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). DS Repositorio Institucional de la Universidad de Burgos RD 04-may-2024