RT info:eu-repo/semantics/article
T1 Commonalities and Differences in the Transcriptional Response of the Model Fungus Saccharomyces cerevisiae to Different Commercial Graphene Oxide Materials
A1 Laguna Teno, Félix
A1 Suarez Diez, Maria
A1 Tamayo Ramos, Juan Antonio
K1 Saccharomyces cerevisiae
K1 Biological response
K1 Commercial graphene oxide
K1 Chelating agent
K1 RNA isolation
K1 Transcriptomics
K1 Differential expression
K1 Microbiología
K1 Microbiology
K1 Materiales
K1 Materials
AB Graphene oxide has become a very appealing nanomaterial during the last years for many different applications, but its possible impact in different biological systems remains unclear. Here, an assessment to understand the toxicity of different commercial graphene oxide nanomaterials on the unicellular fungal model organism Saccharomyces cerevisiae was performed. For this task, an RNA purification protocol was optimized to avoid the high nucleic acid absorption capacity of graphene oxide. The developed protocol is based on a sorbitol gradient separation process for the isolation of adequate ribonucleic acid levels (in concentration and purity) from yeast cultures exposed to the carbon derived nanomaterial. To pinpoint potential toxicity mechanisms and pathways, the transcriptome of S. cerevisiae exposed to 160 mg L–1 of monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) was studied and compared. Both graphene oxide products induced expression changes in a common group of genes (104), many of them related to iron homeostasis, starvation and stress response, amino acid metabolism and formate catabolism. Also, a high number of genes were only differentially expressed in either GO (236) or GOC (1077) exposures, indicating that different commercial products can induce specific changes in the physiological state of the fungus.
PB Frontiers Media
SN 1664-302X
YR 2020
FD 2020-08
LK http://hdl.handle.net/10259/7616
UL http://hdl.handle.net/10259/7616
LA eng
NO This work was supported by the European Union’s H2020 Research and Innovation Programme under the Marie Skłodowska-Curie Grant Agreement Nos. 691095 and 734873 and Junta de Castilla y Leon-FEDER under Grant Nos. BU079U16 and UBU-16-B.
DS Repositorio Institucional de la Universidad de Burgos
RD 23-nov-2024