RT info:eu-repo/semantics/article
T1 Production of small peptides and low molecular weight amino acids by subcritical water from fish meal: Effect of pressurization agent
A1 Barea Gómez, Pedro
A1 Melgosa Gómez, Rodrigo
A1 Illera Gigante, Alba Ester
A1 Alonso Riaño, Patricia
A1 Díaz de Cerio Alonso de Mezquía, Elixabet
A1 Benito Román, Oscar
A1 Beltrán Calvo, Sagrario
A1 Sanz Díez, Mª Teresa
K1 Soluble fish meal protein
K1 Hydrolysis
K1 Subcritical water
K1 Carbon dioxide
K1 Ingeniería química
K1 Chemical engineering
K1 Alimentos
K1 Food
AB The hydrolysis of the water-soluble protein (WSP) fraction from tuna fish meal was evaluated by subcritical water (subW) by using N2 and CO2 as different pressurization agents in the temperature range from 140 to 180 °C. For both gases, the amino group release increased by increasing working temperature while the Lowry response decreased due to production of smaller-size peptides and free amino acids.The free amino acid content was higher with CO2 than with N2. At 180 °C, 344 ± 5 and 275 ± 3 mg of free amino acids per g of WSP were released, respectively; although, in both systems the smallest molecular weight amino acids, glycine and alanine, were preferentially released. The free amino acids content obtained by enzymatic hydrolysis with commercial proteases Alcalase and Novozym was much lower with the highest hydrolysis yield determined for histidine. These results have been supported by size exclusion chromatography analysis.
PB Elsevier
SN 0308-8146
YR 2023
FD 2023-08
LK http://hdl.handle.net/10259/7703
UL http://hdl.handle.net/10259/7703
LA eng
NO This work was supported by the Agencia Estatal de Investigación (AEI) and Ministerio de Ciencia e Innovación (MICINN) [grant numbers PID2019-104950RB-I00 / AEI / https://doi.org/10.13039/501100011033, PID2020-116716RJ-I00/ AEI /https://doi.org/10.13039/501100011033] by the AEI, MICINN, UE Next GenerationEU (Plan de Recuperación, Transformación y Resiliencia) [grant numbers TED2021-129311B-I00, PDC2022-133443-I00] and the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [grant number BU050P20]. Benito Román post-doctoral contract was funded by AEI through project PID2020–116716RJ-I00. P. Alonso-Riaño and P. Barea predoctoral contracts were funded by JCyL and the European Social Fund (ESF) by ORDEN EDU/556/2019, de 5 de junio and by ORDEN EDU/1868/2022, de 19 de diciembre. E Díaz de Cerio and A.E. Illera post-doctoral contracts were funded by JCyL and ERDF through project BU050P20. R. Melgosa contract was funded by a Beatriz Galindo Research Fellowship [BG20/00182].
DS Repositorio Institucional de la Universidad de Burgos
RD 09-may-2024