RT info:eu-repo/semantics/article
T1 Isolation and Quantification of Mandelonitrile from Arabidopsis thaliana Using Gas Chromatography/Mass Spectrometry
A1 Arnáiz Alonso, Ana
A1 Vallejo García, Jorge Lucas
A1 Vallejos Calzada, Saúl
A1 Diaz, Isabel
K1 Benzaldehyde cyanohydrin
K1 Mandelonitrile
K1 Hydroxynitrile lyase
K1 Arabidopsis thaliana
K1 Spider mite
K1 Cyanogenic glycosides
K1 Cyanohydrin
K1 Química orgánica
K1 Chemistry, Organic
AB Mandelonitrile is a nitrogen-containing compound, considered an essential secondary metabolite. Chemically, it isa cyanohydrin derivative of benzaldehyde, with relevant functions in different physiological processes includingdefense against phytophagous arthropods. So far, procedures for detecting mandelonitrile have been effectivelyapplied in cyanogenic plant species such as Prunus spp. Nevertheless, its presence in Arabidopsis thaliana,considered a non-cyanogenic species, has never been determined. Here, we report the development of an accurateprotocol for mandelonitrile quantification in A. thaliana within the context of A. thaliana–spider mite interaction.First, mandelonitrile was isolated from Arabidopsis rosettes using methanol; then, it was derivatized by silylationto enhance detection and, finally, it was quantified using gas chromatography–mass spectrometry. The selectivityand sensitivity of this method make it possible to detect low levels of mandelonitrile (LOD 3 ppm) in a plant speciesconsidered non-cyanogenic that, therefore, will have little to no cyanogenic compounds, using a small quantity ofstarting material (≥ 100 mg).
PB Bio-protocol LLC
SN 2331-8325
YR 2023
FD 2023-06
LK http://hdl.handle.net/10259/7714
UL http://hdl.handle.net/10259/7714
LA eng
NO Protocolo
NO This work was supported by funds of “La Caixa” Foundation (LCF/PR18/51130007) and Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid).
DS Repositorio Institucional de la Universidad de Burgos
RD 24-nov-2024