RT info:eu-repo/semantics/article
T1 Bioaccessibility and Genoprotective Effect of Melanoidins Obtained from Common and Soft Bread Crusts: Relationship between Melanoidins and Their Bioactivity
A1 Temiño, Virginia
A1 Gerardi, Gisela
A1 Cavia Saiz, Mónica
A1 Diaz Morales, Noelia
A1 Muñiz Rodríguez, Pilar
A1 Salazar Mardones, Gonzalo
K1 Melanoidins
K1 Bioaccessible fractions
K1 Membrane permeability
K1 Transepithelial transport
K1 Genoprotective effect
K1 Alimentos
K1 Food
K1 Bioquímica
K1 Biochemistry
AB Bread crust constitutes an important by-product of the bakery industry, and its utilizationfor the isolation of melanoidins to be used as a functional ingredient can enhance its added valueand contribute to health. The aim of this study was to evaluate the bioaccessibility, bioactivity, andgenoprotective effect of melanoidins derived from bread crust. Bioaccessibility was assessed ingastric, intestinal digestion, and colonic fermentation fractions. The results revealed a relationshipbetween bioaccessible melanoidins and their type (common or soft bread). No cytotoxicity effectswere observed for bioaccessible fractions, as assessed by MTT and RTA methods, and they did notaffect the distribution of E-cadherin in Caco-2 cells, confirming their ability to maintain membraneintegrity. Furthermore, our study demonstrated that the gastrointestinal and colonic fermentationfractions successfully transported across the intestinal barrier, without affecting cell permeability, andshowed antioxidant activity on the basolateral side of the cell monolayer. Remarkably, both fractionsdisplayed a significant genoprotective effect in Caco-2 cells. Our findings provide crucial insightsinto the relationship between the melanoidins and their bioactivity and genoprotective effect. Theseresults demonstrated the potential of bioaccessible melanoidins as valuable bioactive compounds forthe development of functional foods, without showing toxic effects on gastrointestinal cells.
PB MDPI
SN 2304-8158
YR 2023
FD 2023
LK http://hdl.handle.net/10259/7952
UL http://hdl.handle.net/10259/7952
LA spa
NO This research was funded by the Autonomous Government of Castilla y Leon and Feder (JCyL/FEDER), grant number BU243P18.
DS Repositorio Institucional de la Universidad de Burgos
RD 09-may-2024