RT info:eu-repo/semantics/article
T1 Isolation and characterization of two glycolipopeptids biosurfactants produced by a Lactiplantibacillus plantarum OL5 strain isolated from green olive curing water
A1 Ammar, Ameni Ben
A1 Bouassida, Mouna
A1 Bouallegue, Amir
A1 Fourati, Nada
A1 Gerardi, Gisela
A1 Muñiz Rodríguez, Pilar
A1 Benito Moreno, José Manuel
A1 Ghribi, Dhouha
K1 Lactobacillus
K1 Biosurfactants
K1 FTIR analysis
K1 Glycolipopeptide
K1 Biología molecular
K1 Molecular biology
K1 Bioquímica
K1 Biochemistry
K1 Alimentos
K1 Food
AB Microbial surfactants are natural amphiphilic compounds with high surface activities and emulsifying properties. Due to their structural diversity, low toxicity, biodegradability, and chemical stability in different conditions, these molecules are potential substitutes for chemical surfactants; their interest has grown significantly over the last decade. The current study focuses on the isolation, identification, and characterization of a lactic acid bacteria that produce two forms of biosurfactants. The OL5 strain was isolated from green olive fermentation and identified using MALDI/TOF and DNAr16S amplification. Emulsification activity and surface tension measurements were used to estimate biosurfactant production. The two biosurfactants derived from Lactiplantibacillus plantarum OL5 presented good emulsification powers in the presence of various oils. They were also shown to have the potential to reduce water surface tension from 69 mN/m to 34 mN/m and 37 mN/m within a critical micelle concentration (CMC) of 7 mg/ml and 1.8 mg/ml, respectively, for cell bound and extracellular biosurfactants. Thin layer chromatography (TLC) and FT-IR were used to analyze the composition of the two biosurfactants produced. the obtained data revealed that the two biomolecules consist of a mixture of carbohydrates, lipids and proteins. We demonstrated that they are two anionic biosurfactants with glycolipopeptide nature which are stable in extreme conditions of temperature, pH and salinity.
PB Springer
SN 0959-3993
YR 2023
FD 2023-11
LK http://hdl.handle.net/10259/8052
UL http://hdl.handle.net/10259/8052
LA eng
NO This work was supported by the Ministry of High Education and Scientific Research- Tunisia.
DS Repositorio Institucional de la Universidad de Burgos
RD 09-may-2024