RT info:eu-repo/semantics/article
T1 Modulation of Akt-p38-MAPK/Nrf2/SIRT1 and NF-κB pathways by wine pomace product in hyperglycemic endothelial cell line
A1 Gerardi, Gisela
A1 Cavia Saiz, Mónica
A1 Rivero Pérez, Maria Dolores
A1 González San José, Mª Luisa
A1 Muñiz Rodríguez, Pilar
K1 Polyphenols
K1 Wine pomace
K1 Endothelial dysfunction
K1 NF-κB
K1 Nrf2
K1 p38MAPK
K1 Akt
K1 SIRT1
K1 Oxidative stress
K1 EA.hy926
K1 Phase 2 enzymes
K1 SOD2
K1 NOX4
K1 COX2
K1 Alimentos
K1 Food
K1 Bioquímica
K1 Biochemistry
K1 Biología molecular
K1 Molecular biology
AB Wine by-products show great potential as source of bioactive compounds that protect the vascular endothelial function by the modulation of both Nrf2 and Nf-κB pathways. This study investigates the pathways involved in the effects of a red wine pomace product (rWPP) against inflammatory and oxidative damage in hyperglycemic EA.hy926 endothelial cells. rWPP-digested fractions showed an inhibitory effect on IKK/IκBα/NF-κB pathway and a stimulatory effect on Akt-p38-MAPK/Nrf2 pathway with an impact on their antioxidant and anti-inflammatory downstream targets. In addition, the pathways regulation was also accompanied by downregulation of the gene expression of superoxide dismutase 2, cyclooxygenase 2 and NADPH oxidase 4. These results suggest the expression of SOD2 as an early adaptive response to the inflammatory effect mediated by NF-κB in hyperglycemic cells, and the treatment with the rWPP-digested fractions regulate this inflammatory process by Nrf2 pathway increased expression of antioxidant enzymes.
PB Elsevier
SN 1756-4646
YR 2019
FD 2019-07
LK http://hdl.handle.net/10259/8054
UL http://hdl.handle.net/10259/8054
LA spa
NO The authors thank the financial support of the Autonomous Government of Castilla y León (Research project BU282U13).
DS Repositorio Institucional de la Universidad de Burgos
RD 24-nov-2024