RT info:eu-repo/semantics/article
T1 Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy
A1 Durán Fernández-Feijóo, Cristina
A1 Rodríguez-Fanjul, Javier
A1 López-Abat, Miriam
A1 Hadley, Stephanie
A1 Cavia Saiz, Mónica
A1 Muñiz Rodríguez, Pilar
A1 Arnaez, Juan
A1 Fernández-Lorenzo, José Ramón
A1 Camprubí Camprubí, Marta
K1 Allopurinol
K1 Hypothermia
K1 Hypoxic-ischemic encephalopathy
K1 Oxidative stress
K1 Oxidative damage
K1 Bioquímica
K1 Biochemistry
K1 Biología molecular
K1 Molecular biology
AB Hypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidativedamage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Ouraim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day ratpups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated,hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia+ allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH/GSSG ratio andcarbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma andcerebrospinal fluid, and 8-iso-prostaglandin F2α was measured in brain tissue. Plasma 2,20–azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that receivedallopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferricreducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantlyreduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotectionin HIE.
PB MDPI
YR 2021
FD 2021-09
LK http://hdl.handle.net/10259/8069
UL http://hdl.handle.net/10259/8069
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 10-may-2024